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Updated: Jul 26, 2025

Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
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Fast, precise and cloning-free knock-in of reporter sequences in vivo with high efficiency

Yiran Zhang1, Katy Marshall-Phelps1, Rafael Góis de Almeida1

  • 1Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, EH16 4SB, UK.

Development (Cambridge, England)
|June 13, 2023
PubMed

Keywords:
HDRHomology-directed repairKnock-inNeuronSynaptobrevinVampZebrafish

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