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Related Experiment Video

Updated: Jul 26, 2025

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High-Throughput SARS-CoV-2 Antiviral Testing Method Using the Celigo Image Cytometer.

Laura A St Clair1,2, Leo Li-Ying Chan3, Adam Boretsky4

  • 1Center for Vector-borne Infectious Diseases, Department of Microbiology, Immunology, and Pathology, Colorado State University, CO, 80523, Fort Collins, USA.

Journal of Fluorescence
|June 13, 2023
PubMed
Summary

Researchers developed a rapid, high-throughput antiviral testing method using the Celigo Image Cytometer. This new approach significantly reduces testing time for identifying effective SARS-CoV-2 antivirals against emerging variants.

Keywords:
AntiviralCOVID-19CeligoDrug repurposingImage cytometrySARS-CoV-2

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Area of Science:

  • Virology
  • Drug Discovery
  • Biotechnology

Background:

  • The COVID-19 pandemic caused a global health crisis, necessitating rapid development of vaccines and antivirals.
  • Emerging SARS-CoV-2 variants of concern require continuous discovery of new antiviral drug candidates with high efficacy.
  • Traditional antiviral testing methods like plaque-reduction neutralization tests (PRNTs) and plaque assays are time-consuming and labor-intensive.

Purpose of the Study:

  • To develop a high-throughput antiviral testing method for SARS-CoV-2 using the Celigo Image Cytometer.
  • To assess the efficacy of antiviral drug candidates against SARS-CoV-2 infectivity and their safety on host cells.
  • To reduce the processing time for antiviral drug screening compared to conventional methods.

Main Methods:

  • Utilized a fluorescent reporter virus to monitor SARS-CoV-2 infectivity.
  • Employed the Celigo Image Cytometer for high-throughput screening of antiviral drug candidates.
  • Assessed cytotoxicity of drug candidates on human cell lines using fluorescent viability stains.

Main Results:

  • The developed method significantly reduced antiviral testing time by an average of 3-4 days.
  • Successfully utilized human cell lines directly, which are not typically suitable for PRNT or plaque assays.
  • Demonstrated the Celigo Image Cytometer's capability for efficient and robust antiviral screening.

Conclusions:

  • The Celigo Image Cytometer provides an efficient and robust platform for rapid antiviral drug discovery against SARS-CoV-2 and its variants.
  • This high-throughput method accelerates the identification of potential antiviral drugs needed during the ongoing pandemic.
  • The approach allows for direct testing on relevant human cell lines, improving the biological relevance of antiviral screening.