Regulation of whole-transcriptome sequencing expression in COPD after personalized precise exercise training: a pilot...

Area of Science:

• Pulmonary Medicine
• Genomics
• Exercise Physiology

Background:

• Chronic obstructive pulmonary disease (COPD) is a leading global cause of mortality.
• Aerobic exercise is crucial for pulmonary rehabilitation in COPD patients.
• Limited research exists on exercise-induced transcriptomic changes in COPD.

Purpose of the Study:

• To investigate RNA transcript level changes in COPD patients after 12 weeks of aerobic exercise.
• To construct RNA networks and explore crosstalk between transcripts.
• To identify potential regulatory mechanisms of exercise in COPD.

Main Methods:

• High-throughput RNA sequencing of peripheral blood from COPD patients pre- and post-aerobic exercise.
• Analysis of mRNA, miRNA, lncRNA, and circRNA expression.
• Construction of coexpression and competing endogenous RNA (ceRNA) networks.

Main Results:

• Identified 86 mRNAs, 570 lncRNAs, 8 miRNAs, and 2087 circRNAs differentially expressed post-exercise.
• Differentially expressed RNAs correlate with biological processes like chemotaxis, DNA replication, and immunometabolism.
• Validated RNA sequencing findings and constructed ceRNA networks.

Conclusions:

• Transcriptomic profiling provides a systematic understanding of exercise's impact on COPD.
• Identified potential RNA candidates for elucidating exercise's regulatory mechanisms in COPD.
• Enhanced understanding of COPD pathophysiology through exercise-induced transcriptomic changes.