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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
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Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

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Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
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Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

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An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a low-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.
To...
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Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
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Recent Developments in Mass Spectrometry to Support Next-Generation Synthesis and Screening.

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New analytical techniques, particularly mass spectrometry, are crucial for accelerating drug discovery. This review covers advanced mass spectrometry workflows for faster screening and synthesis of novel therapeutics.

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Area of Science:

  • Pharmaceutical Science
  • Analytical Chemistry
  • Medicinal Chemistry

Background:

  • Drug discovery timelines are shrinking amid increasing therapeutic complexity.
  • Novel analytical techniques are essential for efficient drug discovery and development.
  • Mass spectrometry is a key analytical technique utilized throughout the drug discovery pipeline.

Purpose of the Study:

  • To review the application and implementation of new mass spectrometry workflows.
  • To highlight how these workflows support current and future drug discovery efforts.
  • To address the demand for faster analytical methods in drug development.

Main Methods:

  • Review of recent advancements in mass spectrometry instrumentation.
  • Analysis of novel sampling and method development in mass spectrometry.
  • Exploration of mass spectrometry applications in drug screening and synthesis workflows.

Main Results:

  • New mass spectrometers and methods are evolving to match modern drug discovery demands.
  • Mass spectrometry workflows facilitate quicker screening and synthesis of potential therapeutics.
  • The integration of advanced mass spectrometry enhances efficiency across the drug discovery pipeline.

Conclusions:

  • Advanced mass spectrometry workflows are vital for meeting the accelerating demands of drug discovery.
  • Continuous innovation in mass spectrometry supports the development of novel therapeutics.
  • Implementing new mass spectrometry techniques is key to future drug screening and synthesis success.