Related Experiment Video

Updated: Jul 26, 2025

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

3.3K

Deciphering glioma epitranscriptome: focus on RNA modifications

Christina Piperi1, Mariam Markouli2, Antonios N Gargalionis3

  • 1Department of Biological Chemistry, Medical School, National and Kapodistrian University of Athens, Athens, Greece. cpiperi@med.uoa.gr.

Oncogene
|June 15, 2023
PubMed

Insights

RNA modifications are crucial in glioma development and treatment resistance. Understanding these changes offers new therapeutic strategies for brain tumors.

Area of Science:

  • Oncology
  • Molecular Biology
  • Immunology

Background:

  • Gliomas are aggressive brain tumors with poor treatment outcomes.
  • Standard therapies often face challenges due to tumor aggressiveness and resistance.
  • Understanding glioma biology is key to developing effective treatments.

Purpose of the Study:

  • To review RNA modifications in glioma progression and tumor microenvironment (TME) immunoregulation.
  • To summarize advances in targeting RNA modifications for glioma therapy.
  • To explore the role of RNA modifications in adaptive drug resistance.

Main Methods:

  • Literature review of recent studies on RNA modifications in gliomas.
  • Analysis of research on RNA modification targeting strategies.
  • Synthesis of information on TME immunoregulation and drug resistance.

Main Results:

  • RNA modifications play a significant role in glioma tumorigenesis and progression.
  • Specific RNA modifications influence tumor microenvironment immunoregulation.
  • These modifications are implicated in the development of adaptive drug resistance.

Conclusions:

  • Targeting RNA modifications presents a promising avenue for novel glioma therapies.
  • Further research into RNA modification mechanisms can improve treatment efficacy.
  • Understanding these molecular mechanisms is vital for overcoming therapeutic challenges.

More Related Videos

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.7K
Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.4K

Related Concept Videos

RNA Editing02:23

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.1K
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.3K
Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.2K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.8K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K