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Updated: Jul 26, 2025
Establishing a Porcine Ex Vivo Cornea Model for Studying Drug Treatments against Bacterial Keratitis
Published on: May 12, 2020
Microbial keratitis and antibiotic sensitivity patterns: A retrospective analysis at a tertiary center in Central
Gautam Singh Parmar1, Ashok Kumar Meena, Prashant Borde
1Department of Cornea and Refractive Surgery, Sadguru Netra Chikitsalaya and Postgraduate Institute of Ophthalmology, Chitrakoot, Madhya Pradesh, India.
Purpose:
To report on the microbiological profile and antibiotic sensitivity patterns of infectious keratitis at a tertiary center in central India.
Methods:
The suspected case of severe keratitis underwent microbiological culture and identification using the VITEK 2 technique. Antibiotic susceptibility for different sensitivity and resistance patterns was analyzed. Demographics, clinical profile, and socioeconomic history was also documented.
Results:
Culture was positive in 233/455 (51.2%) patients. Pure bacterial growth was present in 83 (35.62%) patients and pure fungus was present in 146 (62.66%) patients. The most common bacterial cause of infectious keratitis was Pseudomonas followed by Staphylococcus and Bacillus. Pseudomonas showed 65%-75% resistance against levofloxacin, ceftazidime, imipenem, gentamycin, ciprofloxacin, and amikacin. Staphylococcus showed 65%-70% resistance against levofloxacin, erythromycin, and ciprofloxacin, with Streptococcus being 100% resistant to erythromycin.
Conclusion:
This study highlights the current trend of microbiological profiles of infectious keratitis and their antibiotic susceptibility at a rural setup in central India. Fungal predominance and increased resistance against the commonly used antibiotics were noted.
