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Solubility of Hydrophobic Compounds in Aqueous Solution Using Combinations of Self-assembling Peptide and Amino Acid
Published on: September 20, 2017
16.9K
Rationally designed modular drug delivery platform based on intracellular peptide self-assembly.
Hong-Wei An1, Muhetaerjiang Mamuti1, Xiaofeng Wang2
1CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety National Center for Nanoscience and Technology (NCNST) Beijing China.
Exploration (Beijing, China)
|June 16, 2023
Summary
Researchers developed a modulated drug-delivery system (MDS) using intracellular peptide self-assembly. This system effectively delivers drugs to target cells, enhancing biodistribution and accumulation for improved therapeutic outcomes.
Area of Science:
- Biomolecular Engineering
- Nanotechnology
- Drug Delivery Systems
Background:
- Modulated molecular design enables intracellular self-assembly for optimized drug delivery, improving biodistribution and metabolism.
- Modular design concepts offer multifunctionality, enhancing drug pharmacokinetics through selectivity and universality.
- Controlling in situ self-assembly for precise drug delivery remains a significant challenge in rational molecular design.
Purpose of the Study:
- To fabricate and evaluate a modulated drug-delivery system (MDS) utilizing intracellular peptide self-assembly for effective drug delivery.
- To investigate the molecular mechanisms governing the self-assembly process and its impact on drug delivery efficacy.
- To demonstrate the targeted delivery and intracellular self-assembly of MDS within cancer cells.
Main Methods:
- Fabrication of a modulated drug-delivery system (MDS) based on a five-functional-motif modular designing strategy.
- Induction of in situ self-assembly into fibrous nanostructures within target cells via caspase3/7 hydrolysis.
- Evaluation of MDS construction and delivery efficacy using experimental studies and molecular simulations.
Main Results:
- MDS successfully self-assembled into fibrous nanostructures intracellularly, directed by caspase3/7 hydrolysis.
- Molecular simulations and experiments revealed that solvent-exposed surface area and non-covalent interactions dictate molecular solubility and self-assembly.
- The system demonstrated adaptability for both hydrophilic and hydrophobic drugs due to structural differences.
- MDS specifically recognized targeted cancer cell lines, leading to prolonged drug retention and accumulation.
Conclusions:
- The developed modulated drug-delivery system (MDS) effectively utilizes intracellular peptide self-assembly for targeted drug delivery.
- Rational molecular design and accurate control over self-assembly are crucial for optimizing drug delivery platforms.
- This strategy holds promise for enhancing drug biodistribution, pharmacokinetics, and therapeutic efficacy in cancer treatment.
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