Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

HIVIL: A human <i>in vitro</i> inflammatory liver model recapitulates immune-associated drug effects with high predictivity.

NAM journal·2026

Same author

Direct contact between iPSC-derived macrophages and hepatocytes drives reciprocal acquisition of Kupffer cell identity and hepatocyte maturation.

eLife·2026

Same author

Nur77 agonism invigorates Natural Killer cell immunity against hepatocellular carcinoma.

Nature communications·2026

Same author

Transcriptomic profiling of epigenetic regulators and metabolic reprogramming in human cholangiocarcinoma.

Frontiers in cell and developmental biology·2026

Same author

A guide for establishing patient-derived organoids from bile samples obtained during endoscopic procedures and performing gene expression knockdown.

Frontiers in cell and developmental biology·2026

Same author

Label-free interferometry platform for drug response profiling of bioprinted tumor organoids at single-organoid resolution.

Nature protocols·2026

Same journal

Ptychography at all wavelengths.

Nature reviews. Methods primers·2026

Same journal

Droplet-based bioprinting.

Nature reviews. Methods primers·2026

Same journal

Laser capture microdissection.

Nature reviews. Methods primers·2026

Same journal

Extracellular vesicle analysis.

Nature reviews. Methods primers·2026

Same journal

In vivo microelectrode arrays for neuroscience.

Nature reviews. Methods primers·2026

Same journal

Light-based vat-polymerization bioprinting.

Nature reviews. Methods primers·2025

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 26, 2025

Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality

Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality

Published on: May 5, 2022

Organoids.

Zixuan Zhao¹, Xinyi Chen², Anna M Dowbaj³

¹Mechanobiology Institute, National University of Singapore, Singapore.

Nature Reviews. Methods Primers

|June 16, 2023

Summary

This summary is machine-generated.

Organoid engineering creates in vitro models mimicking human tissues for research and medicine. This primer details methods for robust organoid development, addressing current limitations and future priorities.

More Related Videos

Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors

Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors

Published on: December 11, 2021

Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function

Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function

Published on: July 29, 2021

Related Experiment Videos

Last Updated: Jul 26, 2025

Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality

Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality

Published on: May 5, 2022

Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors

Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors

Published on: December 11, 2021

Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function

Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function

Published on: July 29, 2021

Area of Science:

Biotechnology
Tissue Engineering
Regenerative Medicine

Background:

Organoids are advanced in vitro models replicating in vivo tissue complexity.
They offer powerful tools for studying human development, regeneration, and disease.
Applications span diagnostics, drug discovery, and personalized medicine.

Purpose of the Study:

To review organoid engineering strategies and materials.
To guide the control of cellular/tissue niches for structure and function.
To outline standards for data quality and reproducibility.

Main Methods:

Utilizing pluripotent or tissue-resident stem cells for organoid derivation.
Implementing various engineering strategies for culture, growth, and maturation.
Selecting appropriate matrices, soluble factors, and physical cues.

Main Results:

Organoid engineering enables recapitulation of native tissue structure and function.
Methods allow for controlled manipulation of the cellular microenvironment.
Key considerations for robust organoid generation are identified.

Conclusions:

Organoids are versatile tools with significant potential in biomedical research.
Addressing limitations in cell isolation, matrix selection, and physical cues is crucial.
Future priorities include refining organoid engineering for broader applications.