Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

78
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
78
CRISPR01:59

CRISPR

52.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.5K
Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

19.5K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
19.5K
What is Genetic Engineering?00:49

What is Genetic Engineering?

74.4K
Overview
74.4K
Transgenic Plants02:50

Transgenic Plants

7.3K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.3K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.1K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Development of conditions for determination of imidazole derivatives in the blood using chromatography-mass spectrometry].

Sudebno-meditsinskaia ekspertizaยท2025
Same author

A comparative study on germination of wheat grains with different anthocyanin pigmentation of the pericarp in natural or induced aging.

Vavilovskii zhurnal genetiki i selektsiiยท2024
Same author

Metabolomic prof iles of Ribes nigrum L. and Lonicera caerulea L. from the collection of the N.I. Vavilov Institute in the setting of Northwest Russia.

Vavilovskii zhurnal genetiki i selektsiiยท2022
Same author

[Genome Editing in Species of the Tribe Triticeae with the CRISPR/Cas System].

Molekuliarnaia biologiiaยท2022
Same author

[Anthocyanins as functional food components].

Vavilovskii zhurnal genetiki i selektsiiยท2021
Same author

Genotyping of potato samples from the GenAgro ICG SB RAS collection using DNA markers of genes conferring resistance to phytopathogens.

Vavilovskii zhurnal genetiki i selektsiiยท2021

Related Experiment Video

Updated: Jul 26, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

2.5K

[Improvement of Crops Using the CRISPR/Cas System: New Target Genes].

Y V Ukhatova1,2, M V Erastenkova1, E S Korshikova1

  • 1Vavilov All-Russian Institute of Plant Genetic Resources, St. Petersburg, 190000 Russia.

Molekuliarnaia Biologiia
|June 16, 2023
PubMed
Summary

This study catalogs target genes for CRISPR/Cas crop improvement, revealing 287 genes edited for yield, quality, and resistance. New target gene editing is expanding crop breeding potential.

Keywords:
CRISPR/Casbiotechnologycultivated plantsdirected mutagenesisgenome editingtarget genes

More Related Videos

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
12:59

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Published on: April 30, 2016

18.0K
Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
06:37

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Published on: July 1, 2021

4.5K

Related Experiment Videos

Last Updated: Jul 26, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

2.5K
High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
12:59

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Published on: April 30, 2016

18.0K
Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
06:37

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Published on: July 1, 2021

4.5K

Area of Science:

  • Agricultural Science
  • Molecular Biology
  • Genetics

Background:

  • The CRISPR/Cas system is crucial for crop genome editing, with target gene selection impacting yield, quality, and stress resistance.
  • Previous reviews covered CRISPR/Cas applications up to August 2019.
  • This study focuses on advancements in target gene editing for cultivated plants from August 2019 to March 2022.

Conclusions:

  • CRISPR/Cas technology has significantly advanced crop molecular genetics, enabling targeted improvements.
  • The editing of new target genes is crucial for enhancing crop productivity, disease resistance, and material properties.
  • Agrobacterium-mediated transformation remains the primary delivery method, with base and prime editing gaining traction for precise modifications.