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Methods for Ex Vivo AAV Transduction in Rodent and Human Retinal Explants
Satheesh Kumar1,2,3, Jiang-Hui Wang1, Guei-Sheung Liu4,5,6
1Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne, VIC, Australia.
Methods in Molecular Biology (Clifton, N.J.)
|June 16, 2023
Abstract:
Translational research is heavily dependent on animal models, and reliable disease models are essential for the development of novel therapies. Here, we outline the methods for culturing mouse and human retinal explants. In addition, we show efficient adeno-associated virus (AAV) transduction of the mouse retinal explants to aid the study and development of AAV-based therapeutics against ocular diseases.
