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Updated: Jul 26, 2025

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Environmental ammonia analysis based on exclusive nitrification by nitrifying biofilm screened from natural
Liang Wang1, Wuping Zhou1, Mengchen Zhang1
1School of Biotechnology and Health Sciences, Wuyi University, Jiangmen, 529020, China.
Abstract:
Biofilm-based biological nitrification is widely used for ammonia removal, while hasn't been explored for ammonia analysis. The stumbling block is the coexist of nitrifying and heterotrophic microbes in real environment resulting in non-specific sensing. Herein, an exclusive ammonia sensing nitrifying biofilm was screened from natural bioresource, and a bioreaction-detection system for the on-line analysis of environmental ammonia based on biological nitrification was reported. The nitrifying microbes were aggregated into a nitrifying biofilm through a result-oriented bioresource enrichment strategy. The predominant nitrifying population and progressive surface reaction in the plug flow bioreactor led to the exclusive and exhaustive ammonia biodegradation for the establishment of a novel analytical method. The on-line ammonia monitoring prototype achieved complete biodegradation for determining ammonium nitrogen within 5 min and showed exceptional reliability in long-term real sample measurements without frequent calibration. This work offers a low-threshold natural screening paradigm for developing sustainable bioresource-based analytical technologies.
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