Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ultrasonography

Ultrasonography

Ultrasonography is an imaging technique that uses high-frequency sound waves to visualize the body's internal structures. It is a non-invasive and safe procedure that does not involve the use of ionizing radiation, making it widely used in various medical fields. Ultrasonography is used to study heart function, blood flow in the neck or extremities, certain conditions such as gallbladder disease, and fetal growth and development.
During an ultrasonography procedure, a handheld device called...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Super-Resolution Ultrasound Based Cell Tracking With Polymeric Nanobubbles.

Advanced materials (Deerfield Beach, Fla.)·2026

Same author

Ultrasound Imaging.

Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer·2026

Same author

Microbubble Shell Stiffness Engineering Enhances Ultrasound Imaging, Drug Delivery, and Sonoporation.

Advanced materials (Deerfield Beach, Fla.)·2025

Same author

Advanced ultrasound methods to improve chronic kidney disease diagnosis.

Npj imaging..·2025

Same author

Ultrasound Localization Microscopy for Cancer Imaging.

IEEE transactions on ultrasonics, ferroelectrics, and frequency control·2025

Same author

Super-Resolution Ultrasound: From Data Acquisition and Motion Correction to Localization, Tracking, and Evaluation.

IEEE transactions on ultrasonics, ferroelectrics, and frequency control·2025

Same journal

Limitations of Mono-Exponential Individual Fitting as a Reference Model for Single-Time-Point Dosimetry in [<sup>177</sup>Lu]Lu-PSMA-617 Therapy.

Zeitschrift fur medizinische Physik·2026

Same journal

Determination of long-range resistance from high resolution impedance spectroscopy in human cadaveric heads.

Zeitschrift fur medizinische Physik·2026

Same journal

Patient-specific quality assurance in stereotactic radiotherapy: clinical practice in absence of guidelines - status and new approach from the DGMP working group for physics and technology in stereotactic radiotherapy.

Zeitschrift fur medizinische Physik·2026

Same journal

Potential and challenges of Positron Emission Tomography beyond conventional preclinical and clinical imaging.

Zeitschrift fur medizinische Physik·2026

Same journal

Evaluation of PET/CT Artificial Intelligence Image Reconstructions VS Harmonized Clinical Reconstruction.

Zeitschrift fur medizinische Physik·2026

Same journal

Adaptation of quality control pipeline for Skeletal Muscle 31P MR Spectroscopy at 3T and 7T.

Zeitschrift fur medizinische Physik·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 26, 2025

Two-Dimensional Super-Resolution Visualization of Rat Brain Microvasculature Using Ultrasound Localization Microscopy

Two-Dimensional Super-Resolution Visualization of Rat Brain Microvasculature Using Ultrasound Localization Microscopy

Published on: March 28, 2025

Ultrasound localization microscopy.

Stefanie Dencks¹, Georg Schmitz¹

¹Lehrstuhl für Medizintechnik, Fakultät für Elektrotechnik und Informationstechnik, Ruhr-Universität Bochum, Bochum, Germany.

Zeitschrift Fur Medizinische Physik

|June 16, 2023

Summary

This summary is machine-generated.

Ultrasound Localization Microscopy (ULM) offers super-resolution microvasculature imaging and flow measurements, even at the capillary level. This technique utilizes microbubbles for enhanced visualization, moving from preclinical to clinical use.

Keywords:

Blood flow velocities Contrast agents Localization microscopy Microvasculature Super-resolution Tracking

More Related Videos

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy

Published on: January 7, 2019

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

Published on: June 12, 2021

Related Experiment Videos

Last Updated: Jul 26, 2025

Two-Dimensional Super-Resolution Visualization of Rat Brain Microvasculature Using Ultrasound Localization Microscopy

Two-Dimensional Super-Resolution Visualization of Rat Brain Microvasculature Using Ultrasound Localization Microscopy

Published on: March 28, 2025

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy

Published on: January 7, 2019

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

Published on: June 12, 2021

Area of Science:

Medical Imaging
Biophysics
Ultrasound Technology

Background:

Conventional ultrasound techniques are diffraction-limited, hindering detailed microvasculature visualization.
Existing methods like CEUS and Doppler lack capillary-level resolution.
Ultrasound Localization Microscopy (ULM) emerges as a super-resolution imaging modality.

Purpose of the Study:

To explain the concept and applicability of ULM for microvessel imaging.
To discuss processing steps for ULM implementation.
To review trade-offs in microvasculature reconstruction and 3D imaging.

Main Methods:

ULM localizes single microbubbles (MBs) with sub-pixel precision.
Tracking MBs across image sequences reveals vascular morphology and flow dynamics.
Commercial, clinically approved contrast agents are used.

Main Results:

ULM achieves resolution beyond conventional ultrasound limits.
Enables imaging and flow measurement down to the capillary level.
Quantitative parameters for microvascular changes can be derived.

Conclusions:

ULM demonstrates significant potential for preclinical and clinical applications.
Applications include studying angiogenesis, vascular degeneration, and organ function.
ULM facilitates a deeper understanding of microvascular physiology and pathology.