Raman Spectroscopy and Machine Learning Enables Estimation of Articular Cartilage Structural, Compositional, and...

To differentiate healthy from artificially degraded articular cartilage and estimate its structural, compositional, and functional properties using Raman spectroscopy (RS).

Visually normal bovine patellae (n = 12) were used in this study. Osteochondral plugs (n = 60) were prepared and artificially degraded either enzymatically (via Collagenase D or Trypsin) or mechanically (via impact loading or surface abrasion) to induce mild to severe cartilage damage; additionally, control plugs were prepared (n = 12). Raman spectra were acquired from the samples before and after artificial degradation. Afterwards, reference biomechanical properties, proteoglycan (PG) content, collagen orientation, and zonal (%) thickness of the samples were measured. Machine learning models (classifiers and regressors) were then developed to discriminate healthy from degraded cartilage based on their Raman spectra and to predict the aforementioned reference properties.

The classifiers accurately categorized healthy and degraded samples (accuracy = 86%), and successfully discerned moderate from severely degraded samples (accuracy = 90%). On the other hand, the regression models estimated cartilage biomechanical properties with reasonable error (≤ 24%), with the lowest error observed in the prediction of instantaneous modulus (12%). With zonal properties, the lowest prediction errors were observed in the deep zone, i.e., PG content (14%), collagen orientation (29%), and zonal thickness (9%).

RS is capable of discriminating between healthy and damaged cartilage, and can estimate tissue properties with reasonable errors. These findings demonstrate the clinical potential of RS.