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Study of Experimental Organ Donation Models for Lung Transplantation
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Inflation using hydrogen improves donor lung quality by regulating mitochondrial function during cold ischemia phase.

Le Duan1,2, Lini Quan1,2, Bin Zheng3

  • 1Department of Anesthesiology, the Second Affiliated Hospital of Harbin Medical University, Harbin, China.

BMC Pulmonary Medicine
|June 17, 2023
PubMed
Summary

Hydrogen gas inflation during cold ischemia improves donor lung quality by reducing mitochondrial damage and inflammation. This process enhances mitochondrial function and may be achieved through the Nrf2/HO-1 pathway.

Keywords:
Cold ischemiaDonor lung qualityHydrogenInflammationLung transplantationMitochondrial function

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Area of Science:

  • Transplantation Science
  • Mitochondrial Biology
  • Biomedical Engineering

Background:

  • Mitochondrial dysfunction impairs organ quality and lung transplant outcomes.
  • The role of hydrogen in preserving mitochondrial function in cold-preserved donor lungs is not well understood.

Purpose of the Study:

  • To investigate the protective effects of hydrogen on mitochondrial dysfunction in donor lungs during cold ischemia.
  • To explore the underlying regulatory mechanisms, specifically the Nrf2/HO-1 pathway.

Main Methods:

  • Donor lungs were inflated with oxygen or a hydrogen-oxygen-nitrogen mixture during cold ischemia.
  • Evaluated inflammation, oxidative stress, apoptosis, histology, and mitochondrial function.
  • Assessed the expression of Nrf2 and HO-1.

Main Results:

  • Hydrogen inflation significantly reduced inflammatory response, oxidative stress, and histopathological damage compared to controls.
  • Hydrogen treatment restored mitochondrial structure and function, inhibited anaerobic glycolysis, and increased mitochondrial biosynthesis.
  • Hydrogen showed superior protection against mitochondrial dysfunction and higher Nrf2/HO-1 levels compared to oxygen alone.

Conclusions:

  • Lung inflation with hydrogen during cold ischemia phase may enhance donor lung quality.
  • Hydrogen mitigates mitochondrial damage, oxidative stress, inflammation, and apoptosis.
  • The protective effects are likely mediated by the activation of the Nrf2/HO-1 pathway.