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Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies

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Developmental-stage specific single-cell human embryo dissociation.

Jenna Lammers¹, Sophie Loubersac¹, Laurent David¹

¹Université de Nantes, CHU Nantes, Inserm, Centre de Recherche en Transplantation et Immunologie, UMR 1064, ITUN, 44000 Nantes, France; Service de Médecine et Biologie de la Reproduction, CHU de Nantes, Nantes, France.

|June 18, 2023

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This study details a protocol for single-cell analysis of human embryos. It enables understanding of early embryo development and cell specification through laser dissection and cell processing techniques.

Area of Science:

Developmental Biology
Genomics
Cell Biology

Background:

Single-cell analysis is crucial for understanding human embryo development.
Detailed molecular insights require robust methods for isolating individual embryonic cells.

Purpose of the Study:

To present a standardized protocol for processing human embryos for single-cell analysis.
To facilitate detailed investigation of molecular mechanisms in early human development.

Main Methods:

Human embryos are cultured to the blastocyst stage.
Laser dissection is employed to isolate polar and mural trophectoderm cells.
Cells undergo dissociation, picking, washing, and dispensing for downstream analysis.

Main Results:

Keywords:

Developmental biology Single Cell

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A comprehensive protocol for preparing human embryos for single-cell analysis is established.
The method allows for the precise isolation of specific cell populations from blastocysts.

Conclusions:

This protocol provides a foundation for advancing single-cell studies in human embryology.
It supports research into cell specification and developmental trajectories during human embryonic development.