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Related Concept Videos

Microtubule Associated Proteins (MAPs)01:42

Microtubule Associated Proteins (MAPs)

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Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
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Related Experiment Video

Updated: Jul 26, 2025

An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons
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An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons

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Development of MAPT S305 mutation models exhibiting elevated 4R tau expression, resulting in altered neuronal and

K R Bowles1,2, D A Pugh1,2, C Pedicone1,2

  • 1Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, United States of America.

Biorxiv : the Preprint Server for Biology
|June 19, 2023
PubMed
Summary

Researchers developed novel human induced pluripotent stem cell (iPSC) lines to model 4R tauopathies. These iPSC lines express high levels of 4R tau in neurons and astrocytes, aiding tauopathy research.

Keywords:
4R tauAstrocytesInflammationMAPTMitochondriaSplicingSynapses

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Area of Science:

  • Neuroscience
  • Genetics
  • Cell Biology

Background:

  • Primary tauopathies are linked to 4R tau, but modeling these diseases is difficult due to low 4R tau expression in iPSC-derived neurons.
  • Developing iPSC models that accurately reflect 4R tauopathy requires strategies to increase 4R tau expression.

Conclusions:

  • A novel panel of human iPSC lines with unprecedented 4R tau levels in neurons and astrocytes was developed.
  • These iPSC lines recapitulate tauopathy phenotypes and reveal functional differences between wild-type and mutant 4R tau.
  • The study underscores the importance of MAPT expression in astrocytes and provides a valuable tool for 4R tauopathy research.