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Response of white blood cells to iodipamide ethyl ester particles
Abstract:
The effect of intravenously injected iodipamide ethyl ester (IDE) particles (150 mgl/kg) on white blood cells was studied by light and electron microscopy. The clearance of IDE from rat plasma also was determined by analyzing free IDE particles in a counting chamber. The total white blood cell count remained essentially unchanged up to 40 minutes after the IDE injection, but the polymorphonuclear (PMN) neutrophil count decreased significantly. At 5 minutes postinjection, occasional PMNs contained ingested IDE particles, but by 40 minutes no intracellular particles could be found in the peripheral circulation. In vitro incubation experiments confirmed that human PMNs ingest IDE particles. In electron microscopy, the cells and particles seemed to be morphologically intact. Of the IDE particles counted at 5 minutes postinjection, only 4% remained in plasma at 30 minutes and none at 40 minutes. The decrease in PMN count apparently reflects sequestration of phagocytic cells from the circulation.
Insights
Intravenously injected iodipamide ethyl ester (IDE) particles are rapidly cleared from rat plasma. Polymorphonuclear (PMN) neutrophil counts decrease as these phagocytic cells sequester circulating IDE.
Area of Science:
- Pharmacokinetics
- Hematology
- Cell Biology
Background:
- Iodipamide ethyl ester (IDE) is an intravenously injected substance.
- The impact of IDE particles on white blood cells requires investigation.
Purpose of the Study:
- To investigate the effect of intravenously injected iodipamide ethyl ester (IDE) particles on white blood cells.
- To determine the clearance rate of IDE particles from rat plasma.
Main Methods:
- Light and electron microscopy were used to study white blood cells.
- Rat plasma IDE levels were quantified using a counting chamber.
- In vitro experiments assessed human PMN ingestion of IDE particles.
Main Results:
- Total white blood cell counts remained stable post-IDE injection.
- Polymorphonuclear (PMN) neutrophil counts significantly decreased.
- IDE particles were observed within PMNs at 5 minutes but not at 40 minutes post-injection.
- Plasma IDE levels dropped to 4% at 30 minutes and undetectable at 40 minutes.
- Electron microscopy showed intact cells and particles.
Conclusions:
- The decrease in PMN count is attributed to the sequestration of phagocytic cells from circulation.
- Human PMNs demonstrate the ability to ingest IDE particles in vitro.
- IDE particles are rapidly cleared from the plasma, likely via phagocytosis by PMNs.