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Fabrication of Engineered Vascular Flaps Using 3D Printing Technologies
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Scaffold-free human vascular calcification model using a bio-three-dimensional printer.

Yukiko Nagaishi1,2, Daiki Murata1, Hiromu Yoshizato1,3

  • 1Center for Regenerative Medicine Research, Faculty of Medicine, Saga University, Saga, Japan.

Biofabrication
|June 20, 2023
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Summary

Researchers developed novel bio-3D printed vascular tissues using human cells to model Mönckeberg

Keywords:
atherosclerosis modelbio-3D printerscaffold-freevascular calcification

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Area of Science:

  • Biomedical Engineering
  • Vascular Biology
  • Regenerative Medicine

Background:

  • Atherosclerosis-related diseases pose a growing health burden.
  • Novel research models are crucial for understanding atherosclerosis and developing treatments.
  • Mönckeberg's medial calcific sclerosis is a specific vascular calcification condition requiring better models.

Purpose of the Study:

  • To design and evaluate bio-3D printed vascular-like tubular tissues as a novel research model.
  • To assess the suitability of these tissues for studying Mönckeberg's medial calcific sclerosis.
  • To investigate the calcification process within these engineered tissues.

Main Methods:

  • Bio-3D printing of vascular-like tubular tissues using human aortic smooth muscle cells, endothelial cells, and fibroblasts.
  • Culturing the printed tissues and stimulating calcification using inorganic phosphate (Pi) or calcium chloride.
  • Histological assessment, micro-computed tomography (micro-CT) imaging, and gene expression analysis (RT-qPCR).

Main Results:

  • The bio-3D printed tubular tissues exhibited structural integrity for up to 3 weeks of culture.
  • Calcification was observed within 1 week of culture in the presence of calcification-stimulating factors.
  • Micro-CT confirmed calcium deposition, and RT-qPCR showed increased expression of osteogenic transcription factors.
  • Pi and rosuvastatin administration influenced tissue calcification.

Conclusions:

  • Bio-3D printed vascular-like tubular tissues composed of human cells represent a viable novel research model.
  • These engineered tissues can be used to study Mönckeberg's medial calcific sclerosis.
  • The model allows for the investigation of calcification mechanisms and potential therapeutic interventions.