Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Capture-SELEX-Derived Low-Nanomolar-Affinity Aptamers for Doxorubicin and Inhibition of Cellular Uptake.

ACS chemical biology·2026
Same author

Enzymes, DNAzymes and nanozymes for environmental remediation.

Nanoscale·2026
Same author

LPI alleviates Alzheimer's disease pathology via the GPR55 receptor.

Neuroscience·2026
Same author

Combining G-Quadruplex and Non-Quadruplex Aptamers with Distinct Thermodynamic Driving Forces for Highly Selective Pb<sup>2+</sup> Detection.

ACS sensors·2026
Same author

Affinity sensors for L-lactate and lactate dehydrogenase.

Analytical methods : advancing methods and applications·2026
Same author

Tuning Library Length Revealing High Affinity Melamine Aptamers with Opposite Fluorescence Responses for Highly Selective Detection.

Analytical chemistry·2026

Related Experiment Video

Updated: Jul 26, 2025

Using Graphene Liquid Cell Transmission Electron Microscopy to Study in Situ Nanocrystal Etching
06:18

Using Graphene Liquid Cell Transmission Electron Microscopy to Study in Situ Nanocrystal Etching

Published on: May 17, 2018

17.2K

Robust fully controlled nanometer liquid layers for high resolution liquid-cell electron microscopy.

Tyler S Lott1, Ariel A Petruk1, Nicolette A Shaw1

  • 1The Ultrafast electron Imaging Laboratory (UeIL), Department of Chemistry and Waterloo Institute for Nanotechnology (WIN), University of Waterloo, 200 University Ave. W, N2L 3G1, Waterloo, Ontario, Canada. gsciaini@uwaterloo.ca.

Lab on a Chip
|June 21, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed a new liquid cell electron microscopy (LCEM) method using shape-engineered nanofluidic cells and air-free loading. This technique overcomes window bulging for high-resolution, wide-field imaging of unstained biospecimens in liquid.

More Related Videos

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy
07:37

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy

Published on: December 20, 2012

12.8K
Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles
08:39

Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles

Published on: October 16, 2017

12.8K

Related Experiment Videos

Last Updated: Jul 26, 2025

Using Graphene Liquid Cell Transmission Electron Microscopy to Study in Situ Nanocrystal Etching
06:18

Using Graphene Liquid Cell Transmission Electron Microscopy to Study in Situ Nanocrystal Etching

Published on: May 17, 2018

17.2K
Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy
07:37

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy

Published on: December 20, 2012

12.8K
Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles
08:39

Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles

Published on: October 16, 2017

12.8K

Area of Science:

  • Electron Microscopy
  • Nanotechnology
  • Biophysics

Background:

  • Liquid cell electron microscopy (LCEM) faces challenges with reproducibility and image quality due to window bulging in vacuum.
  • Existing LCEM methods struggle to provide high resolution over a wide field of view.

Purpose of the Study:

  • To introduce a novel LCEM approach that eliminates window bulging for improved imaging.
  • To enable high-resolution, high-throughput observation of unstained biospecimens in their native liquid environment.

Main Methods:

  • Development of a shape-engineered nanofluidic cell architecture.
  • Implementation of an air-free drop-casting sample loading technique.
  • Utilizing these methods to achieve robust, bulgeless imaging conditions.

Main Results:

  • Demonstrated high-resolution imaging across the entire viewing window.
  • Achieved sufficient contrast for observing unstained liposomes.
  • Enabled quantitative measurements of liquid layer thickness.

Conclusions:

  • The new LCEM method provides reproducible, high-quality imaging of in-liquid samples.
  • This technique facilitates high-resolution movies of biospecimens in near-native conditions.
  • The approach opens new avenues for studying dynamic biological processes at the nanoscale.