Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

4.9K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.9K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

74
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
74
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.4K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.4K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

10.7K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
10.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A global effort toward standards for data sharing in biomedical imaging : Developing Consensus and Infrastructure for Global Data Interoperability.

EMBO reports·2025
Same author

MIFA: Metadata, Incentives, Formats and Accessibility guidelines to improve the reuse of AI datasets for bioimage analysis.

Nature methods·2025
Same author

Enabling global image data sharing in the life sciences.

Nature methods·2025
Same author

Rapid generation of homozygous fluorescent knock-in human cells using CRISPR-Cas9 genome editing and validation by automated imaging and digital PCR screening.

Nature protocols·2024
Same author

Facilitating remote and virtual access provision by European research infrastructures - requirements, issues, and recommendations.

Open research Europe·2024
Same author

Intermediate filaments at a glance.

Journal of cell science·2024
Same journal

Dynamic changes in OTULIN and progranulin levels in experimental myocardial infarction and cardiac remodeling.

Histochemistry and cell biology·2026
Same journal

Eosinophil-associated matrix remodeling in a sterile granulomatous inflammation model: a temporal histopathological analysis.

Histochemistry and cell biology·2026
Same journal

Cellular accumulation of lipofuscin in the heart: implications in health and disease.

Histochemistry and cell biology·2026
Same journal

From lipofuscin accumulation to cellular dysfunction: a focus on liver pathophysiology.

Histochemistry and cell biology·2026
Same journal

Immunohistochemical study of α-keratin, loricrin, filaggrin-like protein, and transglutaminase-1 expression in orthokeratinized and parakeratinized epithelium of the tongue of domestic goose (Anser anser f. domestica) during embryonic development.

Histochemistry and cell biology·2026
Same journal

Replication stress induced exposure to methotrexate in root meristem cells of Vicia faba.

Histochemistry and cell biology·2026
See all related articles

Related Experiment Video

Updated: Jul 26, 2025

Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images
14:28

Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images

Published on: July 15, 2020

8.0K

Building a FAIR image data ecosystem for microscopy communities.

Isabel Kemmer1, Antje Keppler1, Beatriz Serrano-Solano1

  • 1Euro-BioImaging ERIC Bio-Hub, European Molecular Biology Laboratory (EMBL) Heidelberg, Meyerhofstraße 1, 69117, Heidelberg, Germany.

Histochemistry and Cell Biology
|June 21, 2023
PubMed
Summary
This summary is machine-generated.

The microscopy community is developing solutions for big data challenges in bioimaging. Efforts focus on harmonized data handling and analysis to achieve FAIR bioimaging data principles.

Keywords:
BioimagingCommunityData managementFAIRMetadata

More Related Videos

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management
10:23

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management

Published on: June 23, 2023

2.8K
Rapid Analysis and Exploration of Fluorescence Microscopy Images
11:41

Rapid Analysis and Exploration of Fluorescence Microscopy Images

Published on: March 19, 2014

12.4K

Related Experiment Videos

Last Updated: Jul 26, 2025

Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images
14:28

Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images

Published on: July 15, 2020

8.0K
Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management
10:23

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management

Published on: June 23, 2023

2.8K
Rapid Analysis and Exploration of Fluorescence Microscopy Images
11:41

Rapid Analysis and Exploration of Fluorescence Microscopy Images

Published on: March 19, 2014

12.4K

Area of Science:

  • Microscopy and Bioimaging

Background:

  • Bioimaging generates large, complex datasets due to rapid advancements in microscopy.
  • Current data handling, analysis, and management practices hinder the full utilization of bioimaging data.

Purpose of the Study:

  • To outline community efforts addressing challenges in FAIR bioimaging data.
  • To highlight collaborative synergies and the role of research infrastructures in advancing bioimaging.

Main Methods:

  • Review of current initiatives and solutions within the microscopy community.
  • Analysis of collaborative efforts among diverse stakeholders in the microscopy ecosystem.

Main Results:

  • Development of strategies and solutions for FAIR bioimaging data.
  • Identification of synergistic approaches and the role of research infrastructures like Euro-BioImaging.

Conclusions:

  • The microscopy community is actively working towards FAIR bioimaging data principles.
  • Collaboration and research infrastructures are crucial for overcoming big data challenges in bioimaging.