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Surface Functional Modification by Ti3 C2 Tx MXene on PLLA Nanofibers for Optimizing Neural Stem Cell Engineering.

Yi-Dan Zhu1, Xi-Ying Ma2, Lin-Peng Li1

  • 1Shanghai Key Laboratory of Sleep Disordered Breathing, Department of Otolaryngology-Head and Neck Surgery, Otolaryngology Institute of Shanghai JiaoTong University, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200233, China.

Advanced Healthcare Materials
|June 21, 2023
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Summary

This study enhances neural stem cell (NSC) growth and differentiation for neurological disease treatment using Ti3C2Tx MXene-coated nanofibers. This biomaterial improves neural regeneration and reduces the body's response to implants.

Keywords:
Ti3C2Tx MXenealigned PLLA nanofibersconductivityneural stem cellssurface modification

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Area of Science:

  • Biomaterials Science
  • Neuroscience
  • Nanotechnology

Background:

  • Optimizing neural stem cell (NSC) substrates is crucial for treating neurological diseases.
  • Current substrates lack the necessary functionality, conductivity, and biocompatibility for efficient neurogenesis.
  • Developing advanced materials for guided neural regeneration remains a significant challenge.

Purpose of the Study:

  • To develop a novel biomaterial by coating aligned poly(l-lactide) (PLLA) nanofibers with Ti3C2Tx MXene.
  • To enhance NSC neurogenesis, direct cell growth, and improve neural regeneration.
  • To investigate the underlying molecular mechanisms and in vivo biocompatibility of the modified substrate.

Main Methods:

  • Surface modification of aligned PLLA nanofibers with Ti3C2Tx MXene.
  • Characterization of substrate properties: conductivity, surface chemistry, hydrophilicity, and topography.
  • In vitro studies on NSC adhesion, proliferation, and differentiation.
  • RNA sequencing analysis to elucidate molecular pathways.
  • In vivo assessment of the foreign body response to implanted PLLA nanofibers.

Main Results:

  • Ti3C2Tx MXene coating endowed PLLA nanofibers with enhanced conductivity, functional groups, hydrophilicity, and roughness.
  • The modified substrate significantly promoted NSC adhesion, proliferation, and differentiation into neurons and astrocytes.
  • Ti3C2Tx MXene synergized with nanofiber alignment to enhance neurite outgrowth and neuronal maturation.
  • RNA sequencing revealed molecular mechanisms of NSC fate modulation by Ti3C2Tx MXene.
  • Surface modification mitigated the in vivo foreign body response to PLLA nanofibers.

Conclusions:

  • Ti3C2Tx MXene-coated aligned PLLA nanofibers represent a promising strategy for neural tissue engineering.
  • This advanced biomaterial offers synergistic benefits for promoting neural regeneration and improving implant biocompatibility.
  • The findings provide a foundation for developing novel therapeutic strategies for neurological disorders.