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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

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Speckle-correlation imaging through a kaleidoscopic multimode fiber.

Dorian Bouchet¹, Antonio Miguel Caravaca-Aguirre¹, Guillaume Godefroy^1,2

¹Université Grenoble Alpes, CNRS, LIPhy, 38000 Grenoble, France.

Proceedings of the National Academy of Sciences of the United States of America

|June 21, 2023

Summary

Researchers developed a new method for noninvasive fluorescence imaging through square-core multimode fibers using speckle correlations. This technique bypasses the need for prior knowledge of the fiber, enabling flexible endoscope development.

Keywords:

light propagation memory effect multimode fibers optical imaging speckle correlations

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Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

Published on: March 20, 2017

Area of Science:

Optics
Biomedical Imaging
Materials Science

Background:

Speckle-correlation imaging enables noninvasive visualization through scattering media.
Image reconstruction in multimode fibers using speckle correlations is a significant challenge.
Multimode fibers and scattering media exhibit analogous light propagation properties.

Purpose of the Study:

To demonstrate fluorescence imaging through square-core multimode fibers without prior knowledge.
To leverage the kaleidoscopic memory effect in square-core fibers for image reconstruction.
To develop a method applicable to flexible, minimally invasive endoscopes.

Main Methods:

Utilizing a kaleidoscopic memory effect in square-core multimode fibers.
Translating random speckle patterns at the fiber input.
Measuring fluorescence intensity with a bucket detector.
Reconstructing images from the signal's autocorrelation via inverse problem solving.

Main Results:

Successful fluorescence imaging through a square-core multimode fiber.
Demonstration of image reconstruction without knowledge of the fiber's transmission matrix.
The method is robust and does not require calibration for the fiber's optical properties.

Conclusions:

The proposed speckle-correlation technique overcomes limitations in fiber imaging.
This approach facilitates the development of novel, flexible endoscopes for minimally invasive procedures.
The method's independence from prior fiber knowledge enhances its practical applicability.