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Exercise and Cardiovascular Response01:20

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Exercise significantly impacts cardiovascular response, which is crucial for understanding patient health and designing effective treatment plans.
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Related Experiment Video

Updated: Jul 26, 2025

Conducting Maximal and Submaximal Endurance Exercise Testing to Measure Physiological and Biological Responses to Acute Exercise in Humans
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Plasma Proteomic Kinetics in Response to Acute Exercise.

Michael Y Mi1, Jacob L Barber2, Prashant Rao1

  • 1Division of Cardiovascular Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA; CardioVascular Institute, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.

Molecular & Cellular Proteomics : MCP
|June 21, 2023
PubMed
Summary
This summary is machine-generated.

Acute exercise significantly alters hundreds of plasma proteins, with many returning to baseline levels within an hour post-exercise. These exercise-induced protein changes may serve as biomarkers for cardiometabolic health.

Keywords:
HERITAGE Family Studyacute exercisecirculationexercise treadmill testplasmaproteomics

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Area of Science:

  • Exercise physiology and proteomics
  • Cardiometabolic disease research
  • Biomarker discovery

Background:

  • Regular exercise benefits human health, potentially through circulating bioactive factors released during physical activity.
  • Limited data exist on plasma protein kinetics during and after acute exercise bouts.
  • Understanding these changes is crucial for identifying exercise-related health mechanisms.

Purpose of the Study:

  • To comprehensively profile plasma proteins during and after acute exercise.
  • To identify proteins associated with cardiometabolic traits and exercise training.
  • To establish a biochemical roadmap of acute exercise effects.

Main Methods:

  • Proteomic profiling of 4163 plasma proteins using affinity-based methods in 75 middle-aged adults undergoing treadmill stress testing.
  • Quantification of plasma proteins at baseline, peak exercise, and 1-hour post-exercise.
  • Examination of protein associations with cardiometabolic traits and changes during a 20-week aerobic exercise intervention.

Main Results:

  • 765 proteins changed significantly at peak exercise, and 128 at 1-hour post-exercise.
  • 56 proteins showed significant changes at both timepoints, including midkine and brain-derived neurotrophic factor.
  • Increased abundance of coagulation-related proteins observed 1-hour post-exercise; 45 proteins linked to cardiometabolic traits, 20 altered by training.

Conclusions:

  • Hundreds of novel plasma proteins change during acute exercise, with most returning to baseline within 1 hour of recovery.
  • Sustained changes in specific proteins during exercise and recovery may indicate valuable circulating biomarkers for cardiometabolic diseases.
  • This study provides a comprehensive biochemical map of acute exercise, available to the scientific community.