Effects of grazing and nitrogen application on greenhouse gas emissions in alpine meadow

Overgrazing and injudicious nitrogen applications have increased emissions of greenhouse gases from grassland ecosystems. To explore the effects and potential mechanisms of grazing, nitrogen application, and their interaction with greenhouse gas (GHG) emissions, field experiments were conducted on the Qinghai-Tibet Plateau for three consecutive years. Alpine meadow plots were subjected to light (8 sheep ha^-1) and heavy (16 sheep ha^-1) stocking rates, with or without ammonium nitrate (NH₄NO₃) (90 kg N ha^-1 yr^-1) treatment to simulate soil nitrogen deposition. During early warm growth season (May-June), peak growth season (July-September), and early cold season (October-November), static-chamber gas chromatography was used to analyze the soil's greenhouse gas emissions (CO₂, N₂O, and CH₄). Results indicated that light stocking rate (LG) led to an increase in cumulative CO₂ and N₂O emissions, while also promoting CH₄ uptake. Conversely, heavy stocking rate (HG) produced contrasting outcomes. Additionally, nitrogen applications significantly increased the short-term CO₂ and N₂O fluxes peaks. Combined treatment of nitrogen application and light stocking rate (LG + N) resulted in increased CO₂ and N₂O emissions while decreased CH₄ uptake, consequently leading to a significant increase in global warming potential. According to the structural equation model, we discovered that nitrogen application and grazing affected GHG fluxes both directly and indirectly through their impact on the environmental factors. Our findings suggest that in the context of increasing nitrogen deposition in the Qinghai-Tibet Plateau, a moderate increase in stocking rate is more effective than reducing grazing intensity for mitigating global warming potential in alpine meadow.