Related Experiment Video

Updated: Jul 26, 2025

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells
08:38

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay PCA in Living Cells

Published on: March 3, 2015

13.4K

Multi-Omic Analysis of CIC's Functional Networks Reveals Novel Interaction Partners and a Potential Role in Mitotic

Yuka Takemon1,2, Véronique G LeBlanc2, Jungeun Song2

  • 1Genome Science and Technology Graduate Program, University of British Columbia, Vancouver, BC V5Z 4S6, Canada.

Cancers
|June 22, 2023
PubMed

Insights

Loss of the CIC tumor suppressor disrupts cell division and RNA splicing. This study reveals CIC’s network interactions and its role in maintaining genomic stability, offering new cancer insights.

Area of Science:

  • * Molecular Biology
  • * Cancer Genomics
  • * Systems Biology

Background:

  • * The CIC gene encodes a transcriptional repressor and MAPK signaling effector, frequently inactivated in cancers, suggesting a tumor suppressor role.
  • * Understanding CIC's functional network is crucial for elucidating its role in tumorigenesis.

Purpose of the Study:

  • * To investigate the interaction networks of CIC using multi-omic approaches.
  • * To characterize the functional consequences of CIC loss in cellular processes like cell cycle regulation and RNA splicing.

Main Methods:

  • * Bioinformatic, genomic, and proteomic analyses to identify CIC interaction partners.
  • * Examination of mitotic defects and gene expression in CIC-deficient cell lines and a mouse model.
  • * Analysis of RNA splicing patterns in CIC-deficient cells.

Main Results:

  • * Identified novel interactions between CIC and SWI/SNF complex members, cell cycle regulators, and RNA processing factors.
  • * CIC loss correlates with increased mitotic defects and dysregulated mitotic regulators.
  • * Aberrant RNA splicing, particularly in untranslated regions, was observed in CIC-deficient cells, affecting key cancer-related genes.

Conclusions:

  • * CIC loss impacts cell cycle regulation, mitotic integrity, and RNA splicing, expanding its known functions.
  • * Multi-omic network analysis effectively uncovers the complex roles of pleiotropic genes like CIC.
  • * This research deepens the understanding of CIC's tumor suppressor functions and potential therapeutic strategies in cancer.
Keywords:
CICcell cyclegenetic networksmitosisproteomic interactionssingle-cell sequencingsplicing

More Related Videos

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

14.3K
Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation00:07

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

Published on: August 21, 2019

8.5K

Related Concept Videos

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.0K
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.6K
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
5.5K