Evaluation of Biosurfactant Production by Bacillus Species Using Glucose and Xylose as Carbon Sources

Lignocellulosic material is one of the raw materials that can be used to reduce the cost of biosurfactant production because it is cheap, abundantly available, and contains cellulose and hemicellulose which can be hydrolyzed to glucose and xylose as carbon sources. This study aimed to evaluate biosurfactant production by Bacillus species using glucose and xylose as carbon sources, which are the most abundant sugar monomers from the hydrolysis of lignocellulosic materials. In this study, biosurfactants were produced by six bacterial isolates belonging to the Bacillus genus. The six bacterial isolates were identified molecularly through 16S rRNA sequencing. The results showed that the six bacterial isolates were identified as B. subtilis ITBCC46, B. subtilis ITBCC40, B. subtilis ITBCC31, B. siamensis ITBCC36, B. xiamenensis ITBCC43, and B. subtilis ITBCC30. All Bacillus species used in this study could be grown on glucose or xylose media. Biosurfactants produced by B. subtilis ITBCC46, B. subtilis ITBCC40, B. subtilis ITBCC31, and B. siamensis ITBCC36 could reduce surface tension below 40 mN/m (32.70 to 39.15 mN/m). All biosurfactants produced by these Bacillus species had more than 50% emulsification stability. These characteristics indicated that the biosurfactants had the desired quality.