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Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli
Published on: November 30, 2018
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Cellular roadmaps of viroid infection
Junfei Ma1, Shachinthaka D Dissanayaka Mudiyanselage2, Jie Hao1
1Department of Biological Sciences, Mississippi State University, Mississippi State, MS 39762, USA; Current address: Plant Pathology Department, University of Florida, Gainesville, FL 32611, USA.
Trends in Microbiology
|June 22, 2023
Summary
Viroids, tiny plant pathogens, are being newly discovered in vast numbers. Understanding their movement within cells offers insights into RNA biology and potential human pathogen research.
Area of Science:
- Molecular Biology
- Virology
- RNA Biology
Background:
- Viroids are single-stranded, circular, noncoding RNAs infecting plants.
- Over 20,000 viroid-like RNA sequences suggest complex biological roles.
- Hepatitis delta virus (HDV), a human pathogen, shares replication mechanisms with viroids.
Purpose of the Study:
- To summarize recent advancements in understanding viroid subcellular trafficking.
- To emphasize the roles of RNA motifs and structural dynamics in viroid replication.
- To compare viroid intracellular trafficking with cellular RNA movement.
Main Methods:
- Review of recent literature on viroid replication and intracellular transport.
- Comparative analysis of viroid trafficking pathways and cellular RNA movement.
- Emphasis on RNA structure-function relationships in viroid biology.
Main Results:
- Viroids shuttle among subcellular compartments to complete replication.
- RNA motifs and structural dynamics critically regulate viroid biological processes.
- Viroid trafficking shares parallels with, yet differs from, known cellular RNA pathways.
Conclusions:
- Understanding viroid intracellular trafficking provides insights into RNA localization and function.
- Research on viroids informs studies on HDV and other viroid-like RNAs.
- Future studies on RNA structures and factors regulating viroid trafficking will advance RNA biology.
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