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Phenotypic and genetic features of a novel clinically isolated rough morphotype Candida auris

Sufei Tian1, Jian Bing2, Yunzhuo Chu1

  • 1National Clinical Research Center for Laboratory Medicine, Department of Laboratory Medicine, The First Hospital of China Medical University, Shenyang, China.

Frontiers in Microbiology
|June 23, 2023
PubMed
Abstract

Insights

A novel rough morphotype of Candida auris, a critical fungal pathogen, has been identified causing urinary tract infections. This discovery offers new insights into the evolution and characteristics of this concerning fungus.

Area of Science:

  • Medical Mycology
  • Infectious Diseases
  • Genomics

Background:

  • Candida auris is a globally significant, emerging pathogenic fungus.
  • It is classified by the World Health Organization (WHO) among the most critical and health-threatening fungi.
  • This study focuses on a newly identified phenotypic variation of C. auris.

Purpose of the Study:

  • To report the first instance of a rough morphotype C. auris strain causing urinary tract infections in non-intensive care unit (ICU) inpatients.
  • To characterize the morphology, genome, and transcriptome of this novel rough morphotype.
  • To compare the rough morphotype with its smooth counterpart.

Main Methods:

  • Phenotypic characterization of the rough morphotype.
  • Scanning Electron Microscopy (SEM) for detailed surface analysis.
  • Whole-genome resequencing and RNA sequencing (RNA-seq) for genetic and transcriptional profiling.

Main Results:

  • A rough morphotype C. auris strain was identified causing UTIs in non-ICU patients.
  • SEM revealed a rough, uneven colony surface with radial wrinkles and irregular spore arrangement.
  • Genomic analysis confirmed genetic similarity to smooth strains but identified five specific SNP mutations; RNA-seq showed differential expression of genes related to cell wall structure and function.

Conclusions:

  • The rough morphotype of C. auris is associated with structural and functional alterations in cell wall proteins.
  • This novel morphotype provides a foundation for future research into the evolutionary dynamics of C. auris.
  • Understanding these variations is crucial for managing C. auris infections.
Keywords:
ACE2Candida aurisPSA31RNA-seqrough morphotype

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