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Double-Staining Method to Detect Pectin in Plant-Fungus Interaction
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Interaction between ultrasound-modified pectin and icaritin.

Yipeng Chen1, Yueming Jiang1, Lingrong Wen1

  • 1State Key Laboratory of Plant Diversity and Prominent Crops, Guangdong Provincial Key Laboratory of Applied Botany, Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; South China National Botanical Garden, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Food Chemistry
|June 24, 2023
PubMed
Summary

Ultrasound-modified pectin (UMP) forms smaller icaritin/pectin micelles (IPMs) with stronger interactions and enhanced anti-cancer activity against HepG2 cells, despite lower initial loading.

Keywords:
Antiproliferative activityEncapsulation efficiencyFlavonoidLoading capacityMicrostructureNMR

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Pharmacology

Background:

  • Pectin enhances icaritin bioaccessibility as a nanocarrier.
  • Ultrasound treatment modifies pectin structure, but its interaction with icaritin is unclear.

Purpose of the Study:

  • Investigate the effects of ultrasound-modified pectin (UMP) on the physiochemical properties of icaritin/pectin micelles (IPMs).
  • Characterize the interaction between UMP and icaritin within IPMs.
  • Evaluate the anti-proliferation activity of UMP-modified IPMs.

Main Methods:

  • Preparation and characterization of native and UMP-modified IPMs.
  • Analysis of particle size, encapsulation efficiency, and loading capacity.
  • Nuclear Magnetic Resonance (NMR) spectroscopy to elucidate hydrogen bonding interactions.
  • Assessment of anti-proliferation activity against HepG2 cells.

Main Results:

  • UMP-IPMs exhibited smaller particle sizes (325-399 nm) compared to native IPMs (551 nm).
  • NMR data revealed stronger hydrogen bond interactions between icaritin and UMP, involving 7-OH of icaritin.
  • UMP-IPMs showed enhanced anti-proliferation activity against HepG2 cells.

Conclusions:

  • Ultrasound modification alters pectin structure, influencing IPM characteristics.
  • Stronger hydrogen bonding in UMP-IPMs enhances icaritin's anti-cancer efficacy.
  • UMP represents a promising approach for developing improved icaritin nanocarriers.