Magnetophoretic circuits: A review of device designs and implementation for precise single-cell manipulation

Area of Science:

• Biotechnology
• Microfluidics
• Cellular Engineering

Background:

• Lab-on-a-chip (LOC) technology is crucial for advancements in biology and medicine.
• Precise manipulation of single particles and cells on-chip is essential for quantitative analysis.
• Existing methods require improvement for high-throughput, controlled single-cell manipulation.

Purpose of the Study:

• To review the principles, manufacturing, and simulation of magnetophoretic circuits.
• To provide a tutorial for operating magnetophoretic devices with various circuit elements.
• To compare magnetophoretic circuits with other microchip platforms for cellular manipulation.

Main Methods:

• Review of theoretical considerations for magnetophoretic circuit design and simulation.
• Detailed operational tutorial for magnetophoretic devices.
• Comparative analysis of magnetophoretic circuits against other microfluidic manipulation techniques.

Main Results:

• Magnetophoretic circuits, inspired by electrical systems, provide parallel and controlled manipulation of numerous bioparticles.
• These circuits utilize passive and active elements for enhanced control and automation.
• The review covers manufacturing, simulation, and operational aspects of these devices.

Conclusions:

• Magnetophoretic circuits offer a unique solution for critical bioanalytical applications.
• This technology addresses unmet needs in single-cell biology and medicine by enabling precise cellular analysis.
• Further development and application of magnetophoretic circuits are expected to drive innovation in the field.