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Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

3.1K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.1K

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Related Experiment Video

Updated: Jul 25, 2025

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

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DNA Methylation Analysis.

Naoko Hattori1, Yu-Yu Liu1, Toshikazu Ushijima2

  • 1Division of Epigenomics, Institute for Advanced Life Sciences, Hoshi University, Tokyo, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|June 24, 2023
PubMed
Summary
This summary is machine-generated.

DNA methylation analysis reveals critical information about cell states and disease. Protocols are provided for bisulfite conversion, BeadChip arrays, and quantitative methylation-specific PCR for accurate DNA methylation detection.

Keywords:
Bisulfite sequencingBisulfite-mediated DNA conversionDNA methylationInfiniumMethylation-specific PCR

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Last Updated: Jul 25, 2025

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Targeted DNA Methylation Analysis by Next-generation Sequencing
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Methodology for Accurate Detection of Mitochondrial DNA Methylation
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Area of Science:

  • Epigenetics and Molecular Biology
  • Genomics and Bioinformatics

Background:

  • DNA methylation regulates gene expression, impacting cellular function and disease states.
  • Alterations in DNA methylation patterns are informative for tissue origin, disease risk, and patient prognosis.
  • Bisulfite conversion is a key method for detecting DNA methylation, though it has limitations.

Purpose of the Study:

  • To provide comprehensive protocols for DNA methylation analysis.
  • To detail methods for bisulfite conversion and various downstream analyses.
  • To enable accurate detection and interpretation of DNA methylation status.

Main Methods:

  • Bisulfite conversion of DNA to differentiate methylated and unmethylated cytosines.
  • Genome-wide analysis using BeadChip microarrays or next-generation sequencing (NGS).
  • Region-specific analysis via methylation-specific PCR (MSP) and bisulfite sequencing.

Main Results:

  • Established protocols for bisulfite conversion, Infinium BeadChip arrays, quantitative MSP, and bisulfite sequencing.
  • Demonstrated the utility of these methods for analyzing DNA methylation.
  • Highlighted the importance of accurate DNA methylation detection for biological insights.

Conclusions:

  • Accurate DNA methylation analysis is crucial for understanding gene regulation and disease.
  • The provided protocols offer reliable methods for both genome-wide and region-specific DNA methylation studies.
  • These techniques facilitate the interpretation of DNA methylation data for various applications.