Headspace single drop microextraction based visual colorimetry for highly sensitive, selective and matrix...

Excessive intake of SO₂, a widely-used food additive, is able to cause respiratory, cardiovascular and neurological disease. For effectively monitoring SO₂ level, we have developed a headspace single drop microextraction based visual colorimetry for highly sensitive and selective sensing of SO₂ with TMB (3,3',5,5'-tetramethylbenzidine) as a classic chromogenic reagent. A combination of single drop and headspace microextraction integrated merits of high extraction efficiency, low consumption of reagents and excellent matrix interference-resistant ability. The colorimetric principle was based on oxidation of TMB, and SO₂ could compete with TMB to preferentially react with ·OH, resulting in the fading of color blue that could be easily read out by naked eye. LOD was calculated to be 0.53 μM and 5 μM by UV-vis and naked eye, respectively. The method was successfully utilized to analysis of food samples, and the experimental device was miniaturized and easy to construct, thus showing a promising potential in field analysis.