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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jul 25, 2025

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

Published on: August 26, 2021

A method for analyzing programmed cell death in xylem development by flow cytometry.

Ying-Li Liu¹, Ying-Hua Guo², Xue-Qin Song³

¹State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China.

Frontiers in Plant Science

|June 26, 2023

Summary

This summary is machine-generated.

Researchers developed a new flow cytometry method to study programmed cell death (PCD) in woody plants. This technique effectively distinguishes living, early, and late PCD cells, aiding wood formation research.

Keywords:

FACS PCD gene expression woody plants xylem development

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Author Spotlight: A Streamlined Approach to Studying Cell Death Initiation in Hypersensitive Response

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Published on: November 10, 2023

Area of Science:

Plant Biology
Cell Biology
Wood Science

Background:

Programmed cell death (PCD) is crucial for plant development, including wood formation.
Studying PCD in woody plants is challenging due to a lack of efficient methods.
Flow cytometry is common for mammalian apoptosis but underutilized in plants.

Purpose of the Study:

To establish an efficient method for studying programmed cell death (PCD) in woody plants.
To adapt flow cytometry techniques for analyzing plant cell death.
To facilitate research into the molecular mechanisms of wood formation.

Main Methods:

Isolated xylem cell protoplasts from poplar stems.
Stained protoplasts with fluorescein annexin V-FITC and propidium iodide (PI).
Utilized fluorescence-activated cell sorting (FACS) for cell analysis.

Main Results:

Successfully distinguished living, early PCD, and late PCD cells using FACS.
Quantitative analysis of cell populations was achieved.
Gene expression patterns correlated with cell morphology and death stages.

Conclusions:

A novel FACS method is effective for studying PCD in woody plants.
This method provides a valuable tool for investigating wood formation.
The technique enhances our understanding of plant developmental processes.