Neurotropin increases in vitro life span of human fibroblasts

Fibroblasts isolated from human fetal tissues (skin, lung and heart) exhibited the following population doubling levels (PDL): about 40 PDL for skin; 60 PDL for lung; 10 PDL for heart. Neurotropin extract from vaccinia-virus infected skin tissues of rabbits increases the growth rate of the fibroblast when added to the old lung cell culture (PDL 40, 70% of the maximum life span) but not when added to the young lung (PDL 5, 8% of the maximum life span). Neurotropin (40 or 80 micrograms/ml) increased the replicative life span of the three lines of fibroblasts (skin, lung and heart) when added to the young cell cultures. The increase of the maximum PDL of skin, lung and heart fibroblasts with the Neurotropin treatment was by 19%, 5% and 17%, respectively. When Neurotropin was added to the old lung cell culture (PDL 44, 73% of the maximum life span), the maximum PDL was increased by 9%. Since the existence of hydrocortisone, known to extend the in vitro life span, was negligible in the fluorimetric test of the medium containing Neurotropin, this agent may belong to the class other than hydrocortisone, for increasing in vitro life span.