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Related Experiment Videos

A simple method for counting Staphylococcus aureus in swimming pool water.

A Mates, M Schaffer

    Microbios
    |January 1, 1986
    PubMed
    Summary

    Swimming pool water can harbor Staphylococcus aureus, a bacterium detected using membrane filtration. Its presence may not correlate with coliform bacteria, highlighting a potential public health concern.

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    Area of Science:

    • Microbiology
    • Environmental Health
    • Water Quality Analysis

    Background:

    • Swimming pool water is a potential reservoir for pathogenic bacteria.
    • Standard water quality tests often focus on coliforms and fecal coliforms.
    • The presence of Staphylococcus aureus in recreational water poses a health risk.

    Purpose of the Study:

    • To quantify Staphylococcus aureus in swimming pool water.
    • To investigate the correlation between Staphylococcus aureus and indicator bacteria (coliforms, fecal coliforms).
    • To assess the reliability of thermonuclease and coagulase tests for identifying Staphylococcus aureus.

    Main Methods:

    • Membrane filtration technique was used to filter water samples.
    • Baird-Parker media and nutrient agar were utilized for initial bacterial growth.
    • Selective incubation temperatures and toluidine blue agar overlay were employed for Staphylococcus aureus identification.
    • Thermonuclease and coagulase tests were performed to confirm bacterial presence.

    Main Results:

    • Staphylococcus aureus was detected in swimming pool water samples.
    • The presence of Staphylococcus aureus did not consistently correlate with the presence of coliform or fecal coliform bacteria.
    • Thermonuclease formation was found to correlate with coagulase formation, indicating Staphylococcus aureus.

    Conclusions:

    • Swimming pool water can contain Staphylococcus aureus even in the absence of coliforms.
    • Standard coliform testing may not be sufficient to assess the microbiological safety of swimming pools.
    • Reliable methods exist for detecting Staphylococcus aureus in recreational water environments.

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