A Novel Fluorescent Probe for the Detection of Hydrogen Peroxide

Hydrogen peroxide (H₂O₂) is one of the important reactive oxygen species (ROS), which is closely related to many pathological and physiological processes in living organisms. Excessive H₂O₂ can lead to cancer, diabetes, cardiovascular diseases, and other diseases, so it is necessary to detect H₂O₂ in living cells. Since this work designed a novel fluorescent probe to detect the concentration of H₂O₂, the H₂O₂ reaction group arylboric acid was attached to the fluorescein 3-Acetyl-7-hydroxycoumarin as a specific recognition group for the selective detection of hydrogen peroxide. The experimental results show that the probe can effectively detect H₂O₂ with high selectivity and measure cellular ROS levels. Therefore, this novel fluorescent probe provides a potential monitoring tool for a variety of diseases caused by H₂O₂ excess.