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A biologist's guide to planning and performing quantitative bioimaging experiments
Rebecca A Senft1, Barbara Diaz-Rohrer1, Pina Colarusso2
1Imaging Platform, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, United States of America.
Plos Biology
|June 27, 2023
Summary
Quantitative bioimaging offers powerful insights but requires expertise. This guide helps biologists navigate complex experiments from sample prep to data interpretation for rigorous, reproducible results.
Area of Science:
- * Biological sciences
- * Microscopy and imaging technologies
Background:
- * Advancements in biology and microscopy enable quantitative bioimaging.
- * Increasing experiment complexity necessitates specialized expertise for rigor and reproducibility.
- * Experimental biologists require guidance to adopt quantitative bioimaging effectively.
Purpose of the Study:
- * To provide a navigational guide for experimental biologists on quantitative bioimaging.
- * To enhance understanding of the entire quantitative bioimaging workflow.
- * To empower biologists to conduct rigorous and reproducible quantitative bioimaging experiments.
Main Methods:
- * Discussion of sample preparation, image acquisition, image analysis, and data interpretation.
- * Highlighting the interconnectedness of each step in the bioimaging workflow.
- * Providing general recommendations and key questions for each stage.
Main Results:
- * Offers a synthesis of information for planning and executing quantitative bioimaging experiments.
- * Includes links to high-quality, open-access resources for further learning.
- * Empowers biologists with the knowledge to improve experimental design and execution.
Conclusions:
- * Quantitative bioimaging is transitioning from observational to a more rigorous, data-driven approach.
- * This guide serves as a crucial resource for biologists seeking to master quantitative bioimaging techniques.
- * Efficient execution of complex quantitative bioimaging experiments is achievable with proper guidance.
