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Updated: Jul 25, 2025
Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR
Published on: February 19, 2020
Quantification of hematopoietic stem and progenitor cells by targeted DNA methylation analysis
Ledio Bocova1,2, Wouter Hubens1,2, Cordula Engel3
1Institute for Stem Cell Biology, University Hospital of RWTH Aachen, Aachen, Germany.
Abstract:
Hematopoietic stem and progenitor cells (HSPCs) are quantified in daily clinical practice by flow cytometry. In this study, we provide proof of concept that HSPCs can also be estimated by targeted DNA methylation (DNAm) analysis. The DNAm levels at three individual CG dinucleotides (CpG sites) in the genes MYO1D, STK17A, and SP140 correlated with CD34+ cell numbers in mobilized peripheral blood and with blast counts in leukemia. In the future, such epigenetic biomarkers can support the evaluation of stem cell mobilization, HSPC harvesting, or blast count in leukemia.
