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Related Concept Videos

Phosphoinositides and PIPs01:42

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Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
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Because many receptor binding ligands are hydrophilic, they do not cross the cell membrane and thus their message must be relayed to a second messenger on the inside. There are several second messenger pathways, each with their own way of relaying information. G-protein coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol path is active when the receptor induces phospholipase C to hydrolyze the phospholipid,...
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Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
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Updated: Jul 25, 2025

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
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Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

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Multiple Inositol Polyphosphate Phosphatase Compartmentalization Separates Inositol Phosphate Metabolism from

Jia Yu1, Barbara Leibiger1, Shao-Nian Yang1

  • 1The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital, SE-171 76 Stockholm, Sweden.

Biomolecules
|June 28, 2023
PubMed
Summary
This summary is machine-generated.

Multiple inositol polyphosphate phosphatase (MINPP1) normally resides in the ER. Cytosolic MINPP1 unexpectedly dephosphorylates the growth-signaling lipid phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), explaining its ER localization.

Keywords:
PIP3inositol lipidinositol phosphatemultiple inositol polyphosphate phosphatase (MINPP1)pancreatic beta cellphosphatidylinositol 3,4,5-trisphosphate

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Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
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Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
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Area of Science:

  • Cell Biology
  • Biochemistry
  • Enzymology

Background:

  • Multiple inositol polyphosphate phosphatase (MINPP1) metabolizes inositol phosphates within the endoplasmic reticulum (ER).
  • The ER localization of MINPP1 is poorly understood, but previous studies noted reduced cell growth when MINPP1 was expressed in the cytosol.
  • MINPP1 shares substrate similarities with lipid-metabolizing enzymes like PTEN, suggesting potential roles beyond inositol phosphates.

Purpose of the Study:

  • To investigate the potential lipid phosphatase activity of cytosolic MINPP1 (cyt-MINPP1).
  • To determine if cyt-MINPP1 affects phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), a key signaling lipid in cell growth.
  • To elucidate the functional significance of MINPP1's ER compartmentalization.

Main Methods:

  • Expressing MINPP1 in the cytosol of insulin-secreting HIT cells.
  • Measuring levels of PtdIns(3,4,5)P3 in cells expressing cyt-MINPP1.
  • Assessing the in vitro dephosphorylation of synthetic PtdIns(3,4,5)P3 by purified cyt-MINPP1.

Main Results:

  • Cytosolic expression of MINPP1 led to reduced levels of PtdIns(3,4,5)P3 in HIT cells.
  • MINPP1 demonstrated direct dephosphorylation activity against synthetic PtdIns(3,4,5)P3 in vitro.
  • These findings suggest MINPP1 can impact both inositol phosphate and inositol lipid metabolism.

Conclusions:

  • MINPP1 possesses lipid phosphatase activity, capable of dephosphorylating PtdIns(3,4,5)P3.
  • The ER localization of MINPP1 is crucial for segregating inositol phosphate metabolism from inositol lipid signaling pathways.
  • This compartmentalization ensures distinct regulation of cell growth and inositol phosphate homeostasis.