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Updated: Jul 25, 2025
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A Technique for Serial Collection of Cerebrospinal Fluid from the Cisterna Magna in Mouse
Published on: November 10, 2008
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A Minimally-Invasive Method for Serial Cerebrospinal Fluid Collection and Injection in Rodents with High Survival
Jingrong Regina Han1,2,3,4,5, Yu Yang6, Tianshu William Wu2,3,4,5,7
1School of Life Sciences, Fudan University, Shanghai 200438, China.
Biomedicines
|June 28, 2023
Summary
Researchers developed a novel, cost-effective method for collecting cerebrospinal fluid (CSF) from rodents. This minimally-invasive technique allows for repeated CSF collection and infusion, aiding central nervous system disease research and drug development.
Area of Science:
- Neuroscience
- Biomedical Engineering
- Animal Research
Background:
- Cerebrospinal fluid (CSF) is crucial for diagnosing central nervous system (CNS) diseases.
- Existing methods for CSF collection and infusion in rodents are technically difficult and have high mortality rates.
Purpose of the Study:
- To present a cost-effective and efficient method for accessing CSF in live rodents.
- To enable both CSF collection and infusion for research purposes.
- To improve upon existing protocols for rodent CSF manipulation.
Main Methods:
- A specialized, uniquely angled and lengthed metal needle tool was designed.
- The tool facilitates access to CSF via the foramen magnum in rodents.
- The procedure is minimally invasive, allowing for iterative sample collection.
Main Results:
- Successful collection of 5-10 µL CSF from mice and 70-100 µL from rats.
- High success rates for infusing solutions into the cisterna magna of mice.
- High postoperative recovery rates observed in treated animals.
- Enabled iterative CSF collection from the same animal every few days.
Conclusions:
- The developed protocol provides an efficient and minimally-invasive approach for CSF collection and infusion in rodents.
- This method is expected to advance biomarker discovery and drug development for CNS diseases.
- Facilitates downstream analyses such as mass spectrometry.
