Solid Lipid Nanoparticles Containing Morin: Preparation, Characterization, and Ex Vivo Permeation Studies

Area of Science:

• Nanotechnology
• Materials Science
• Pharmacology

Background:

• Bioactive compounds offer low toxicity but suffer from poor solubility and bioavailability.
• Solid lipid nanoparticles (SLNs) are emerging as advanced drug delivery systems to overcome these limitations.

Purpose of the Study:

• To prepare and characterize morin (MRN)-loaded SLNs (MRN-SLNs) using Compritol® 888 ATO (COM) or Phospholipon® 80H (PHO).
• To evaluate the physical-chemical, morphological, and technological properties of MRN-SLNs for improved drug delivery.

Main Methods:

• MRN-SLNs were prepared via solvent emulsification/diffusion using COM or PHO lipids.
• Characterization included physical-chemical, morphological, encapsulation efficiency, in vitro release, and ex vivo permeation studies.
• Spectroscopic and thermal analyses confirmed MRN-lipid interactions.

Main Results:

• Spherical, non-aggregated MRN-SLNs (60-70 nm) with negative zeta potentials were obtained.
• High encapsulation efficiency (~99%) was achieved, especially with a 10% theoretical MRN load.
• In vitro studies showed ~60% MRN release in 24h with sustained release over 10 days.
• Ex vivo studies demonstrated SLNs as penetration enhancers for MRN across bovine nasal mucosa.

Conclusions:

• MRN-SLNs prepared with COM or PHO exhibit favorable characteristics for drug delivery.
• The developed SLNs enhance morin's encapsulation and release profile.
• SLNs show potential as effective penetration enhancers for nasal drug delivery.