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Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...

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Related Experiment Video

Updated: Jun 18, 2026

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
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Novel Multi-Responsive Hyperbranched Polyelectrolyte Polyplexes as Potential Gene Delivery Vectors.

Dimitrios Selianitis1, Hector Katifelis2, Maria Gazouli2

  • 1Theoretical and Physical Chemistry Institute, National Hellenic Research Foundation, 48 Vassileos Constantinou Avenue, 11635 Athens, Greece.

Pharmaceutics
|June 28, 2023
PubMed
Summary

This study shows that pH and thermo-responsive hyperbranched copolymers effectively bind with DNA, forming nanoscale polyplexes. These non-toxic polyplexes demonstrate excellent stability and potential for gene delivery applications.

Keywords:
MTT assaygene deliveryhyperbranched polyelectrolyte copolymerspH/thermo-sensitivepolyplexesshort DNA

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Area of Science:

  • Polymer Chemistry
  • Biomaterials Science
  • Nanotechnology

Background:

  • Hyperbranched polyelectrolyte copolymers, specifically poly(oligo(ethylene glycol)methyl methacrylate)-co-poly(2-(diisopropylamino)ethyl methacrylate) (P(OEGMA-co-DIPAEMA)), are synthesized using reversible addition fragmentation chain transfer (RAFT) polymerization.
  • These copolymers are designed with varying chemical compositions to investigate their complexation with short-linear DNA molecules.

Purpose of the Study:

  • To explore the complexation behavior of P(OEGMA-co-DIPAEMA) hyperbranched copolymers with linear DNA.
  • To analyze the formation and properties of DNA polyplexes influenced by copolymer composition, N/P ratio, and environmental stimuli (pH, temperature, ionic strength).
  • To evaluate the potential of these polyplexes for gene delivery applications, including their stability and cytotoxicity.

Main Methods:

  • Reversible addition fragmentation chain transfer (RAFT) polymerization for copolymer synthesis.
  • Dynamic and electrophoretic light scattering (DLS, ELS) to characterize polyplex size and charge.
  • Fluorescence spectroscopy (FS) to study complexation dynamics.
  • In vitro cytotoxicity assays on HEK 293 cell lines.

Main Results:

  • P(OEGMA-co-DIPAEMA) hyperbranched copolymers successfully formed nanoscale DNA polyplexes across different N/P ratios.
  • Polyplex characteristics (mass, size) were modulated by copolymer hydrophobicity and N/P ratio.
  • Polyplexes exhibited excellent stability in the presence of serum proteins.
  • In vitro studies demonstrated sufficient non-toxicity of the polyplexes on HEK 293 cells.

Conclusions:

  • The synthesized multi-responsive hyperbranched copolymers are effective in forming stable, nanoscale DNA polyplexes.
  • These polyplexes show tunable properties in response to pH, temperature, and ionic strength.
  • The low cytotoxicity and excellent stability suggest significant potential for these polyplexes in gene delivery and other biomedical applications.