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Controlling the Evolution of Selective Vancomycin Resistance through Successful Ophthalmic Eye-Drop Preparation of
El Tahra M Ahmed1, Mariam Hassan2,3, Rehab Nabil Shamma4
1Department of Pharmaceutics, College of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and Technology, 6th of October City, Giza 12585, Egypt.
Pharmaceutics
|June 28, 2023
Summary
This study enhances vancomycin loading into liposomes using an ammonium sulfate gradient, improving its effectiveness against methicillin-resistant Staphylococcus aureus (MRSA) and preventing resistance development.
Area of Science:
- Pharmacology
- Nanotechnology
- Infectious Diseases
Background:
- Vancomycin is crucial for treating serious methicillin-resistant Staphylococcus aureus (MRSA) infections.
- Suboptimal vancomycin use and emerging resistance threaten its efficacy.
- Nanovesicles offer potential for improved drug delivery but face challenges with vancomycin loading.
Purpose of the Study:
- To develop an effective method for loading vancomycin into liposomes.
- To enhance the therapeutic efficacy of vancomycin against MRSA.
- To investigate the potential of liposome-encapsulated vancomycin in combating vancomycin resistance.
Main Methods:
- Utilized an ammonium sulfate gradient method for vancomycin loading into liposomes.
- Optimized pH conditions (extraliposomal pH 9, intraliposomal pH 5-6) for active vancomycin encapsulation.
- Characterized vancomycin-loaded liposomes for size (155 nm) and entrapment efficiency (up to 65%).
Main Results:
- Achieved high vancomycin entrapment efficiency (up to 65%) in liposomes.
- Demonstrated a 4.6-fold decrease in the minimum inhibitory concentration (MIC) for MRSA.
- Successfully inhibited and killed heteroresistant vancomycin-intermediate S. aureus (h-VISA).
- Observed no development of resistance in MRSA against liposome-delivered vancomycin.
Conclusions:
- Ammonium sulfate gradient method effectively enhances vancomycin loading into liposomes.
- Vancomycin-loaded nanoliposomes significantly improve antibacterial activity and overcome resistance.
- This nanoliposomal drug delivery system presents a viable strategy to enhance vancomycin therapy and combat resistance.
