Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Stability01:53

RNA Stability

33.7K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.7K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

5.7K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
5.7K
Abnormal Proliferation02:23

Abnormal Proliferation

4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

951
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
951
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

7.1K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibition of RNA splicing is a novel therapeutic strategy for disruption of nuclear replicating viruses.

bioRxiv : the preprint server for biology·2026
Same author

Expanding the human proteome with microproteins and peptideins.

Nature·2026
Same author

An expanded reference catalog of translated open reading frames for biomedical research.

Nucleic acids research·2026
Same author

NSD3 stabilizes nuclear compartmentalization and promotes megabase-scale chromatin interactions.

bioRxiv : the preprint server for biology·2026
Same author

InspectorORF: a tool for visualizing Ribo-Seq and additional genomic or transcriptomic data.

Bioinformatics advances·2026
Same author

The dynamic interplay between viruses and host non-coding RNA species.

FEMS microbiology reviews·2026

Related Experiment Video

Updated: Jul 25, 2025

Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy
09:03

Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy

Published on: January 18, 2018

7.1K

m6A Regulates the Stability of Cellular Transcripts Required for Efficient KSHV Lytic Replication.

Oliver Manners1, Belinda Baquero-Perez2, Timothy J Mottram1

  • 1School of Molecular and Cellular Biology, Faculty of Biological Sciences and Astbury Centre of Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK.

Viruses
|June 28, 2023
PubMed
Summary

The study reveals that N6-methyladenosine (m6A) RNA modification is vital for Kaposi's sarcoma-associated herpesvirus (KSHV) replication. It stabilizes GPRC5A mRNA, which regulates viral lytic replication through NF-κB signaling.

Keywords:
GPCR5AKSHVRNA modificationcell signallinglytic replicationm6A methylation

More Related Videos

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

3.3K
In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
08:58

In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells

Published on: May 1, 2019

15.1K

Related Experiment Videos

Last Updated: Jul 25, 2025

Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy
09:03

Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy

Published on: January 18, 2018

7.1K
Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

3.3K
In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
08:58

In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells

Published on: May 1, 2019

15.1K

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Virology

Background:

  • N6-methyladenosine (m6A) is a key epitranscriptomic modification in mammals, regulating mRNA fate and cellular processes.
  • Kaposi's sarcoma-associated herpesvirus (KSHV) infection involves dynamic changes in m6A modification patterns on viral and host mRNAs.
  • KSHV reactivation from latency is a critical step in viral pathogenesis.

Purpose of the Study:

  • To investigate the role of m6A in cellular transcripts during KSHV lytic replication.
  • To elucidate the function of GPRC5A mRNA, upregulated during KSHV infection.
  • To understand how m6A-mediated regulation of cellular genes impacts viral replication.

Main Methods:

  • Analysis of m6A modification patterns in KSHV-infected cells.
  • Quantification of GPRC5A mRNA stability and expression.
  • Investigation of GPRC5A's role in KSHV lytic replication and NF-κB signaling pathways.

Main Results:

  • m6A modification is essential for the stability of GPRC5A mRNA, which is induced by KSHV's RTA protein.
  • GPRC5A expression is upregulated during KSHV lytic replication.
  • GPRC5A directly regulates NF-κB signaling, promoting efficient KSHV lytic replication.

Conclusions:

  • m6A plays a critical role in stabilizing cellular GPRC5A mRNA during KSHV infection.
  • GPRC5A is a key cellular factor essential for KSHV lytic replication, mediated through NF-κB signaling.
  • This study underscores the significance of epitranscriptomic regulation in viral-host interactions.