Related Experiment Video
Updated: Jul 25, 2025
07:23
Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury
Published on: March 7, 2022
6.1K
Riboflavin protects against heart failure via SCAD-dependent DJ-1-Keap1-Nrf2 signalling pathway
Qingping Xu1,2, Yuhong Cao1,2, Xiaoyi Zhong1,2
1School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou, China.
British Journal of Pharmacology
|June 28, 2023
Summary
Riboflavin protects the heart in heart failure by boosting flavin adenine dinucleotide (FAD) and short-chain acyl-CoA dehydrogenase (SCAD) activity. This activates the DJ-1-Keap1-Nrf2 pathway, reducing oxidative stress and cell death.
Area of Science:
- Cardiovascular Research
- Molecular Biology
- Biochemistry
Background:
- Flavin adenine dinucleotide (FAD) demonstrates cardiovascular protective effects through short-chain acyl-CoA dehydrogenase (SCAD) supplementation.
- The role of riboflavin, a FAD precursor, in mitigating heart failure requires further elucidation.
Purpose of the Study:
- To investigate if riboflavin can improve heart failure.
- To determine if riboflavin activates SCAD and the DJ-1-Keap1-Nrf2 signaling pathway for cardioprotection.
Main Methods:
- Utilized a mouse model of transverse aortic constriction (TAC)-induced heart failure.
- Assessed cardiac structure, function, energy metabolism, and apoptosis.
- Analyzed signaling proteins in vivo and in vitro using cell apoptosis models.
Main Results:
- Riboflavin treatment improved cardiac function, ameliorated fibrosis, and reduced apoptosis in TAC mice.
- In vitro studies showed riboflavin decreased reactive oxygen species (ROS) and cardiomyocyte apoptosis.
- Riboflavin restored FAD, SCAD activity, activated DJ-1, and modulated the Keap1-Nrf2/HO1 pathway.
Conclusions:
- Riboflavin exerts cardioprotective effects against heart failure.
- Mechanism involves FAD-mediated stimulation of SCAD, activating the DJ-1-Keap1-Nrf2 pathway.
- This pathway improves oxidative stress and reduces cardiomyocyte apoptosis.
Keywords:
cell apoptosisenergy metabolismheart failureoxidative stressriboflavinshort-chain acyl-CoA dehydrogenasesignalling pathwayMore Related Videos
07:40Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy
Published on: May 26, 2023
1.2K
08:15Network Pharmacology and Validation of the Antidepressant Mechanisms of Qiangzhifang in a Chronic Restraint Stress-induced Depression Rat Model
Published on: June 6, 2025
105
Related Concept Videos
01:26Heart Failure Drugs: Inhibitors of Renin-Angiotensin System
467
The activation of the sympathetic nervous system and the renin-angiotensin-aldosterone system (RAAS) contributes to cardiac remodeling, and inhibiting the RAAS is a pharmacological target in heart failure management. As a result, neurohumoral modulation is a crucial treatment principle for managing heart failure. This approach involves using medications like ACE inhibitors (ACEIs), angiotensin receptor blockers (ARBs), β-blockers, mineralocorticoid receptor antagonists (MRAs), and neutral...
467
01:29Heart Failure II: Pathophysiology
16
Systolic Heart Failure and Compensatory MechanismsSystolic heart failure (also termed HFrEF, Heart Failure with Reduced Ejection Fraction) is the most prevalent type of heart filure. It results in a decreased volume of blood being pumped from the ventricle. The aortic arch and carotid sinuses have baroreceptors that detect reduced blood pressure, triggering the sympathetic nervous system (SNS) to release epinephrine and norepinephrine. Initially, this response aims to boost heart rate and...
16
01:23Transcriptional Regulation: Riboswitches
63
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
63
01:17Pathophysiology of Heart Failure
1.7K
Heart failure (HF) is a progressive syndrome involving ventricles that leads to inadequate cardiac output. It can be classified based on location and output or ejection fraction. Ejection fraction (EF) is an essential measurement in the diagnosis and surveillance of HF. Reduced EF corresponds to systolic heart failure (HFrEF). However, HF with preserved ejection fraction (HFpEF) is becoming increasingly prevalent. Also known as diastolic HF, this form of HF is related to aging. The...
1.7K
01:30Heart Failure V: Medical Management
15
Medical Management of Acute Decompensated Heart Failure (ADHF)The primary goals of therapy for patients hospitalized with acute decompensated heart failure (ADHF) include:Relieving symptomsOptimizing volume statusSupporting oxygenation and ventilationMaintaining cardiac output (CO) and end-organ perfusionIdentifying and addressing the cause of ADHFPreventing complicationsProviding patient education on factors precipitating HF exacerbationPlanning for dischargeOngoing monitoring and assessment...
15
01:22Heart Failure Drugs: Diuretics
425
Heart failure and kidney perfusion are interconnected in a complex way. Reduced renal perfusion and venous congestion are two significant factors that contribute to renal dysfunction in heart failure. The kidneys, primarily responsible for fluid balance in the body, are adversely affected due to compromised cardiac output and increased venous pressure. In response to reduced renal perfusion, the kidneys activate neurohumoral mechanisms to restore balance. However, these mechanisms can be...
425