Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Formation of Complex Ions

Formation of Complex Ions

A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Polychlorinated biphenyl (PCB) exposure assessment of Carolina wren (Thryothorus ludovicianus) and barn swallow (Hirundo rustica) eggs and nestlings collected along Snow Creek and Choccolocco Creek watersheds near Anniston, Alabama, USA.

Environmental toxicology and chemistry·2026

Same author

Reassessing Transketolase Assays: Methodological Considerations for Detecting Functional Thiamine Deficiency.

Annals of the New York Academy of Sciences·2026

Same author

Plasma Biomarkers, Brain Volume, and Cognitive Performance in Service Members and Veterans With mTBI: A LIMBIC-CENC Study.

JAMA network open·2026

Same author

A Noncovalent Click-to-Release Strategy to Control Bond Cleavage and Prodrug Activation.

Angewandte Chemie (International ed. in English)·2026

Same author

Association of Marshall CT Scores with GFAP, UCH-L1, Tau, NfL, and p-Tau231 After Traumatic Brain Injury.

International journal of molecular sciences·2025

Same author

An updated transketolase activity assay to probe thiamine utilization in fish.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP·2025

Search research articles

Related Experiment Video

Updated: Jul 25, 2025

Salinity-dependent Toxicity Assay of Silver Nanocolloids Using Medaka Eggs

Salinity-dependent Toxicity Assay of Silver Nanocolloids Using Medaka Eggs

Published on: March 18, 2016

Understanding and mitigating thiaminase activity in silver carp.

Patricia C Wolfe¹, Amber M Tuske¹, Donald E Tillitt²

¹Department of Pharmaceutical Sciences, Binghamton University, Binghamton, NY, 13902, USA.

Current Research in Food Science

|June 28, 2023

Summary

Thiaminase, an enzyme causing thiamine deficiency, is present in invasive silver carp. Cooking methods like baking and microwaving can reduce its activity, but dehydration and freeze-drying may concentrate it.

Keywords:

Food processing Invasive species Silver carp Thiaminase Thiamine deficiency

More Related Videos

Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function

Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function

Published on: January 22, 2017

A Fish-feeding Laboratory Bioassay to Assess the Antipredatory Activity of Secondary Metabolites from the Tissues of Marine Organisms

A Fish-feeding Laboratory Bioassay to Assess the Antipredatory Activity of Secondary Metabolites from the Tissues of Marine Organisms

Published on: January 11, 2015

Related Experiment Videos

Last Updated: Jul 25, 2025

Salinity-dependent Toxicity Assay of Silver Nanocolloids Using Medaka Eggs

Salinity-dependent Toxicity Assay of Silver Nanocolloids Using Medaka Eggs

Published on: March 18, 2016

Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function

Treating SCA1 Mice with Water-Soluble Compounds to Non-Specifically Boost Mitochondrial Function

Published on: January 22, 2017

A Fish-feeding Laboratory Bioassay to Assess the Antipredatory Activity of Secondary Metabolites from the Tissues of Marine Organisms

A Fish-feeding Laboratory Bioassay to Assess the Antipredatory Activity of Secondary Metabolites from the Tissues of Marine Organisms

Published on: January 11, 2015

Area of Science:

Aquatic Ecology
Food Science
Biochemistry

Background:

Thiamine (vitamin B1) deficiency, caused by the enzyme thiaminase, leads to health issues in various species.
Thiaminase is found in bacteria, plants, and fish, including carp.
Invasive silver carp (Hypophthalmichthys molitrix) are abundant in the Mississippi River watershed and considered a potential food source.

Purpose of the Study:

To confirm the presence of thiaminase in silver carp tissues.
To evaluate the impact of different processing methods (microwaving, baking, dehydrating, freeze-drying) on thiaminase activity.
To assess the implications for silver carp utilization as a food source.

Main Methods:

Thiaminase activity was measured in various silver carp tissues.
The effects of microwaving, baking, dehydration, and freeze-drying on thiaminase activity were systematically examined.
The 4-nitrothiophenol (4-NTP) assay was used to quantify thiaminase activity.

Main Results:

Thiaminase was detected in multiple silver carp tissues, particularly the viscera.
Baking and microwaving at specific temperatures and durations significantly reduced thiaminase activity.
Dehydration and freeze-drying concentrated thiaminase without inactivating it, potentially increasing risks.

Conclusions:

Silver carp contain thiaminase, primarily in their viscera.
Heat-based processing (baking, microwaving) can effectively reduce thiaminase activity.
Non-thermal processing methods like dehydration and freeze-drying require careful consideration due to thiaminase concentration.