Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Mar 25, 2026

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes
10:27

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Published on: May 4, 2018

7.4K

Reassessing Transketolase Assays: Methodological Considerations for Detecting Functional Thiamine Deficiency.

Katie A Edwards1,2

  • 1Department of Pharmaceutical Sciences, School of Pharmacy and Pharmaceutical Sciences, Binghamton University, Binghamton, New York, USA.

Annals of the New York Academy of Sciences
|March 23, 2026
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plasma Biomarkers, Brain Volume, and Cognitive Performance in Service Members and Veterans With mTBI: A LIMBIC-CENC Study.

JAMA network open·2026
Same author

A Noncovalent Click-to-Release Strategy to Control Bond Cleavage and Prodrug Activation.

Angewandte Chemie (International ed. in English)·2026
Same author

Association of Marshall CT Scores with GFAP, UCH-L1, Tau, NfL, and p-Tau231 After Traumatic Brain Injury.

International journal of molecular sciences·2025
Same author

An updated transketolase activity assay to probe thiamine utilization in fish.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP·2025
Same author

Pre-analytical challenges from adsorptive losses associated with thiamine analysis.

Scientific reports·2024
Same author

Serum GFAP, NfL, and tau concentrations are associated with worse neurobehavioral functioning following mild, moderate, and severe TBI: a cross-sectional multiple-cohort study.

Frontiers in neurology·2024
Same journal

Multiomics Profiling During Autoimmune Demyelination Highlights a Complex Regulatory Role for Ataxin-1 in B Cells.

Annals of the New York Academy of Sciences·2026
Same journal

Global Trends in Light Pollution and Their Relationship With Socioeconomic Factors.

Annals of the New York Academy of Sciences·2026
Same journal

Wired for Corruption: Inter-Brain Synchrony Encodes Bribery-Related Value Information and Predicts Bribery Agreement.

Annals of the New York Academy of Sciences·2026
Same journal

LM-YOLO: A Lightweight Multi-Scale Enhanced Model for Forest Smoke Detection Using Unmanned Aerial Vehicles.

Annals of the New York Academy of Sciences·2026
Same journal

Polyrhythm Perception and Production: A Scoping Review.

Annals of the New York Academy of Sciences·2026
Same journal

DARTS-CNN-BiLSTM: Intelligent Fault Diagnosis for Computer Numerical Control Machine Tool Feed System.

Annals of the New York Academy of Sciences·2026
See all related articles

Thiamine (vitamin B1) deficiency is common. Measuring transketolase (TKT) activity, with or without thiamine diphosphate (TDP), offers a functional assessment of thiamine utilization and enzyme function.

Area of Science:

  • Biochemistry
  • Nutritional Science
  • Enzymology

Background:

  • Thiamine (vitamin B1) deficiency is a widespread issue in humans and animals.
  • Thiamine diphosphate (TDP) is a crucial cofactor for metabolic enzymes, notably transketolase (TKT).
  • TKT is a rate-limiting enzyme in the pentose-phosphate pathway, essential for cellular metabolism.

Purpose of the Study:

  • To review preanalytical variables, assay methodologies, and data presentation for TKT measurements.
  • To explore the utility of TKT assays beyond deficiency screening, focusing on enzyme function and cofactor dependence.
  • To provide an improved framework for interpreting TKT measurements as indicators of functional thiamine status.

Main Methods:

  • Evaluation of erythrocyte and tissue TKT activity measurements in human and nonhuman studies.
Keywords:
enzyme assayslatencypentose‐phosphate pathwaythiamine deficiencytransketolasevitamin B1

More Related Videos

Author Spotlight: Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
05:59

Author Spotlight: Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Published on: May 19, 2023

3.7K
Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism
07:59

Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism

Published on: August 19, 2021

4.7K

Related Experiment Videos

Last Updated: Mar 25, 2026

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes
10:27

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Published on: May 4, 2018

7.4K
Author Spotlight: Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals
05:59

Author Spotlight: Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Published on: May 19, 2023

3.7K
Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism
07:59

Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism

Published on: August 19, 2021

4.7K
  • Analysis of strategic variations in assay conditions (e.g., TDP concentration, magnesium presence) to assess TKT function.
  • Review of factors influencing measured TKT activity and responsiveness.

    • Main Results:

    • TKT activity assays, with or without exogenous TDP, provide a functional assessment of thiamine utilization.
    • Assay condition modifications can differentiate true thiamine deficiency from impaired enzyme utilization or altered enzyme properties.
    • Understanding biological and methodological determinants is key to accurate TKT activity interpretation.

    Conclusions:

    • TKT assays offer a valuable, functional measure of thiamine status, complementing direct thiamine quantification.
    • Strategic assay design and interpretation frameworks can enhance the diagnostic utility of TKT measurements.
    • Expanded application of TKT assays can benefit clinical, translational, and ecological studies of thiamine status.