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Updated: Jul 25, 2025

Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
Dataset from genome sequencing, assembly and mining of microsatellite markers in barred-button quail (Turnix
Prateek Dey1,2, Swapna Devi Ray1,2, Padmanabhan Pramod1
1Sálim Ali Centre for Ornithology and Natural History, Anaikatty, Coimbatore, Tamil Nadu 641108, India.
Abstract:
Turnix suscitator (barred-button quail) is a member of the primitive genus Turnix in the highly diverse order of shore birds Charadriiformes. Absence of genome scale data of T. suscitator has limited our understanding about its systematics, taxonomic and evolutionary history as well has hindered the characterization of genome wide microsatellite markers of the same. Hence we generated whole genome short read sequences of T. suscitator, created a high quality assembly and mined genome-wide microsatellite markers from the same. A total of 34142524 reads were sequenced with an estimated genome size of 817 mb. SPAdes assembly consisted of 320761 total contigs and an estimated N50 value of 907 base pairs. Krait identified a total of 77028 microsatellite motifs covering 0.64% of the total sequences in the SPAdes assembly. Further the whole genome sequence and genome wide microsatellites dataset of T. suscitator will facilitate future genomic/evolutionary studies of Turnix species.

