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Published on: March 20, 2015
Structural characterization of exopolysaccharide from Leuconostoc mesenteroides P35 and its binding interaction with
Mojtaba Azari-Anpar1, Kambiz Jahanbin2, Pascal Degraeve3
1Univ Lyon, Université Claude Bernard Lyon 1, ISARA Lyon, BioDyMIA Research Unit, 155 rue Henri de Boissieu, F-01000 Bourg en Bresse, France; Ferdowsi University of Mashhad, Faculty of Agriculture, Department of Food Science and Technology, Mashhad 91775-1163, Iran.
Abstract:
This paper describes the structural elucidation of Leuconostoc mesenteroides P35 exopolysaccharide (EPS-LM). Ln. mesenteroides P35 strain was isolated from a French goat cheese for its capacity to produce EPS increasing the viscosity of a whey-based fermentation medium. The chemical structure of EPS-LM analysis was elucidated by determination of optical rotation degree, macromolecular characterization, sugar units and methylation analyses, FT-IR, 1D NMR spectroscopy (1H and 13C NMR), 2D NMR spectroscopy (1H1H COSY, HSQC and HMBC). EPS-LM was a high molecular weight (ranging from 6.7 × 106 Da to 9.9 × 106 Da) dextran that is composed of only d-glucose units containing α (1 → 6) linkages and paltry α (1 → 3) branches. Since polysaccharide-protein interactions can be exploited to control and design food matrices, EPS-LM interactions with bovine serum albumin (the main constituent of bovine plasma) were investigated by surface plasmon resonance (SPR). Kinetic properties of EPS-LM binding with immobilized BSA via showed an increase of EPS-LM affinity (equilibrium constant (Kd)) for BSA from (2.50 ± 0.01) × 10-5 M-1 at 298 K to (9.21 ± 0.05) × 10-6 M-1 at to 310 K. The thermodynamic parameters revealed that van der Waals and hydrogen binding forces play a major role in the interaction of EPS-LM with BSA. However, EPS-LM-BSA interaction was non-spontaneous, entropy driven and an EPS-LM - BSA binding process was endothermic (ΔG > 0). The structural findings suggested that Ln. mesenteroides P35 α-D-glucan might find widespread technological applications in the biopolymer, medical and food industries.
Insights
This study elucidates the structure of Leuconostoc mesenteroides P35 exopolysaccharide (EPS-LM), a high molecular weight dextran. Researchers found EPS-LM interacts with bovine serum albumin, suggesting potential applications in the biopolymer, medical, and food industries.
Area of Science:
- Microbiology and Biochemistry
- Biopolymer Science
Background:
- Leuconostoc mesenteroides P35 produces exopolysaccharides (EPS) that increase viscosity in whey-based media.
- Exopolysaccharides have potential applications in food matrices due to polysaccharide-protein interactions.
Purpose of the Study:
- To structurally elucidate the exopolysaccharide (EPS-LM) from Leuconostoc mesenteroides P35.
- To investigate the interaction between EPS-LM and bovine serum albumin (BSA).
Main Methods:
- Structural elucidation using techniques including NMR spectroscopy (1D and 2D), FT-IR, and methylation analysis.
- Macromolecular characterization and determination of optical rotation degree.
- Investigation of EPS-LM and BSA interactions using surface plasmon resonance (SPR).
Main Results:
- EPS-LM is a high molecular weight dextran composed of d-glucose units with α (1→6) linkages and α (1→3) branches.
- SPR analysis revealed temperature-dependent affinity of EPS-LM for BSA, with van der Waals and hydrogen binding forces being significant.
- The EPS-LM-BSA binding process was endothermic and entropy-driven, indicating non-spontaneous interaction.
Conclusions:
- The detailed structural characterization of EPS-LM provides a basis for its technological applications.
- The identified interactions with BSA suggest potential uses in the biopolymer, medical, and food industries.
- Further research into EPS-LM properties could unlock novel applications in material science and biotechnology.
