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Extended sample storage for platelet function testing in healthy dogs
Melanie Dickinson1, Anthony Abrams-Ogg1, Shauna L Blois1
1Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Veterinary Clinical Pathology
|June 29, 2023
Summary
Delayed platelet function testing in dogs is feasible using specific methods. Platelet aggregation remained stable for extended periods, especially with AGGFix, allowing for flexible testing schedules.
Area of Science:
- Veterinary Hematology
- Platelet Physiology
Background:
- Platelet function testing is crucial for monitoring antiplatelet therapy.
- Current limitations include time constraints and specialized equipment requirements.
- Delayed testing could improve accessibility and practicality.
Purpose of the Study:
- To evaluate storage methods for canine blood samples.
- To determine the feasibility of delayed platelet function testing.
- To assess the impact of storage on Platelet Function Analyzer (PFA) and Plateletworks (PW) tests.
Main Methods:
- Citrated blood samples from 13 healthy dogs were tested.
- PFA tests used P2Y and CADP cartridges after 2h room temperature, 24h, and 48h refrigeration.
- PW tests analyzed native, room temperature, refrigerated, and AGGFix-preserved samples up to 7 days.
Main Results:
- PFA closure times increased with storage, particularly with the P2Y cartridge.
- Plateletworks aggregation remained robust (88%-94% median) across storage durations.
- AGGFix solution effectively stabilized platelet aggregates for delayed testing.
Conclusions:
- Delayed platelet function testing in dogs is feasible.
- Storage methods and time impact test results, requiring adjusted reference ranges.
- AGGFix shows promise for stabilizing samples for later analysis.
